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Analysis of a eukaryotic β-galactostdase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product

机译:真核β-半乳糖蛋白酶基因的分析:酵母kluyveromyces乳酸乳糜蛋白的N-末端末端显示对大肠杆菌Lacz基因产物的同源性

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The LAC4 gene of Kluyveromyces lactis, encoding the enzyme β-galactosidase was mapped on a cloned DNA fragment and the sequence of the 5′ end was determined. This sequence includes the 5′ regulatory region involved in the induction by lactose and the N-terminal end of the protein coding region. Comparison of the deduced amino acid sequence of this eukaryotic enzyme with the N-terminal end of the Escherichia coil β-galactosidase revealed substantial homology. Two major RNA initiation sites were mapped at -115 and -105. A number of structural peculiarities of the 5′non-coding region are discussed as in comparison to Saccharomyces cerevisiae genes.
机译:kluyveromyces乳酸的LAC4基因,编码酶β-半乳糖苷酶被映射在克隆的DNA片段上,测定5'末端的序列。该序列包括乳糖和蛋白质编码区域的乳糖的诱导中涉及的5'调节区。将这种真核酶的推导氨基酸序列与大肠杆菌β-半乳糖苷酶的N-末端的推导氨基酸序列的比较显示了大量同源性。将两个主要的RNA引发位点映射在-115和-105。与酿酒酵母基因相比,讨论了5'Non编码区域的许多结构特性。

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