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首页> 外文期刊>Journal of Virology >Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses.
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Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses.

机译:硅质病毒40大T抗原的细胞质和核变异型细胞质和核变异的差异磷酸化。

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摘要

The phosphorylation patterns of cytoplasmic and nuclear forms of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses were analyzed by two-dimensional peptide mapping. The PARA(cT) mutant which encodes a large T antigen defective for nuclear transport was used as source for cytoplasmic large T antigen. The data suggest that the large T antigen is phosphorylated in a sequential manner at a subset of sites in the cytoplasm and at additional sites in the nucleus.
机译:通过二维肽测绘分析了Simian病毒40-腺病毒7杂交病毒的辛妇病毒40大T抗原的细胞质和核形式的磷酸化模式。编码核转运缺陷的对大T抗原的对β(CT)突变体被用作细胞质大T抗原的来源。数据表明,大T抗原在细胞质中的位点和在细胞核中的其他位点处以顺序方式磷酸化。

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