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首页> 外文期刊>Journal of Virology >Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion.
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Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion.

机译：Kirsten鼠Sarcoma病毒在转化的非脯氨酸和再转剂NIH / 3T3细胞中的表达：细胞介导对表型逆转中病毒癌基因的验证的证据。

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摘要

Expression of the provirus in a clonally related series of Kirsten murine sarcoma virus-transformed NIH/3T3 nonproducer cell lines was examined at both the transcriptional and translational levels. All cells expressed high levels of genome-sized viral RNA with little variation between cell lines despite differences in provirus integration site and copy number. Expression of K-ras RNA was estimated to be at least 10- to 20-fold higher than that of the mouse cellular homolog of the viral transforming gene. Levels of the virus-coded transforming protein, p21, were similarly elevated, with little variation between nonproducer cells. In two revertant cell lines containing a normal provirus and a rescuable transforming gene, no impairment in expression at either the transcriptional or translational level was found. After superinfection with Kirsten murine sarcoma virus, one revertant became more tumorigenic, whereas the other remained nontumorigenic. These results show that cell transformation by Kirsten murine sarcoma virus is invariably associated with elevated expression of the virus-coded oncogene and that one of the revertants is resistant to the action of the viral transforming gene.
机译：在转录和平移水平的情况下，检查了在克隆相关系列Kironaly相关系列Kironaly相关系列的Kironten鼠肉瘤病毒转化的NIH / 3T3非产蛋白细胞系中的表达。尽管潜意水整合现场和拷贝数存在差异，但所有细胞所有细胞都表达了具有很少的细胞系变异的基因组大小的病毒RNA。估计K-RAS RNA的表达估计比病毒转化基因的小鼠细胞同源物高至少10-20倍。病毒编码的转化蛋白P21的水平类似地升高，无产蛋白细胞之间的变化很小。在含有正常潜水和救援转化基因的两条复位细胞系中，发现在转录或翻译水平的表达中没有损伤。在用Kirsten鼠Sarcoma病毒进行超育后，一个还原剂变得更多的肿瘤，而另一个还留下了Nontumorigenic。这些结果表明，Kirsten鼠Sarcoma病毒的细胞转化总是与病毒编码的癌基因的升高的表达相关，并且还原剂中的一种耐受病毒转化基因的作用。

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