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首页> 外文期刊>Journal of Virology >Envelope gene sequence of two in vitro-generated mink cell focus-forming murine leukemia viruses which contain the entire gp70 sequence of the endogenous nonecotropic parent.
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Envelope gene sequence of two in vitro-generated mink cell focus-forming murine leukemia viruses which contain the entire gp70 sequence of the endogenous nonecotropic parent.

机译：具有两种体外的貂皮细胞聚焦形成鼠白血病病毒的包络基因序列，其含有内源性非渗透母细胞的整个GP70序列。

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摘要

The mink cell focus-forming (MCF) class of recombinant murine leukemia viruses (CI-1 to 4) were isolated from iododeoxyuridine-induced C3H/MCA 5 cells in culture and molecularly cloned. These genomes included infectious (CI-3) and defective (CI-4) recombinants. A total of 2,408 nucleotides of CI-3 virus DNA, including the MCF envelope gene, were sequenced and compared with ecotropic, dual-tropic, and xenotropic sequences. The extent of recombinational exchange in CI-3 was from 145 nucleotides 3' of the splice acceptor site for the envelope mRNA to nucleotide 1,722, between the end of gp70 and the beginning of Prp15E. Thus, the entire gp70 sequence of the endogenous nonecotropic parent was present in this recombinant. The nature and location of the recombinant junctions were consistent with a mechanism involving DNA exchange during reverse transcription. Comparison of the substituted sequence in CI-3 with that of Moloney MCF virus suggests a very close relationship, if not identity, between the endogenous dual-tropic proviruses from which they were derived. A nonidentity of xenotropic and MCF gp70s was observed, suggesting that xenotropic murine leukemia viruses are not the nonecotropic parent of the env gene of MCF murine leukemia viruses. The replication-defective virus CI-4 had a 684-nucleotide deletion present in the env gene, eliminating the hydrophobic regions within the gp70 carboxy end and the p15E amino end. This sequence was bordered by an 11-nucleotide direct repeat in CI-3 viral DNA.
机译：将重组鼠白血病病毒（CI-1至4）的貂皮细胞聚焦形成（MCF）类别与培养物中的碘氧基脲诱导的C3H / MCA 5细胞分离出来。这些基因组包括传染病（CI-3）和缺陷（CI-4）重组剂。总共2,408个核苷酸的CI-3病毒DNA，包括MCF包络基因，并与生态转化，双 - 热带和异熵序列进行比较。 CI-3中的重组交换程度来自接头受体部位的1​​45个核苷酸3'，用于封套mRNA至核苷酸1,722，在GP70和PRP15E的开始之间。因此，在该重组中存在内源性非渗透母细胞的整个GP70序列。重组交界处的性质和位置与涉及在逆转录中的DNA交换的机制一致。与莫洛尼MCF病毒的CI-3中取代序列的比较表明，如果不是身份，则在其衍生的内源性双重热带潜水术之间存在非常紧密的关系。观察到异孔和MCF GP70s的非Intidentity，表明异种鼠白血病病毒不是MCF鼠白血病病毒的ENV基因的非渗透父母。复制缺陷的病毒CI-4具有在环境中存在的684-核苷酸缺失，从而消除了GP70羧基末端和P15E氨基内的疏水区。该序列通过CI-3病毒DNA中的11核苷酸直接重复界定。

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    《Journal of Virology》 |1984年第2期|共10页
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    G E Mark; U R Rapp;

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  • 收录信息 美国《科学引文索引》(SCI);
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