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首页> 外文期刊>The biochemical journal >Degradative inactivation of the peroxisomal enzyme, alcohol oxidase, during adaptation of methanol-grown Candida boidinii to ethanol
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Degradative inactivation of the peroxisomal enzyme, alcohol oxidase, during adaptation of methanol-grown Candida boidinii to ethanol

机译：过氧化合物酶酶，醇氧化酶的降解灭活，适应甲醇生长的念珠菌至乙醇

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摘要

pAdaptation of methanol-grown C. boidinii to ethanol-utilization in non-growing cells resulted in decreased activity of the peroxisomal enzyme alcohol oxidase. Re-appearance of alcohol oxidase activity was dependent on protein synthesis de novo. Degradation of alcohol oxidase protein was shown to parallel the decrease in activity. Adaptation of methanol-grown cells to ethanol-utilization resulted in increased absorbance due to cytochromes and decreased absorbance due to flavoprotein. Decrease in alcohol oxidase activity was associated with loss of the flavin coenzyme, FAD, from the organisms and the appearance of flavins (FAD, FMN, riboflavin) in the surrounding medium. Electron microscopic observations showed that general degradation of whole peroxisomes rather than specific loss of crystalline cores (alcohol oxidase protein) occurred during the adaptation./p
机译：

在非生长细胞中对甲醇生长的C. BoidiniI与乙醇利用的适应导致过氧化物酶酶醇氧化酶的活性降低。醇氧化酶活性的重新出现依赖于蛋白质合成de novo。显示醇氧化酶蛋白的降解并平行于活性的降低。对甲醇生长的细胞对乙醇利用的适应导致含有细胞变色引起的吸光度增加，并且由于黄蛋白引起的吸光度降低。醇氧化酶活性的降低与来自生物体的Flavin辅酶，FAD的丧失与周围培养基中的类生物（FAD，FMN，Riboflavin）的外观相关。电子显微镜观察结果表明，在适应期间发生全过氧血清酶的一般性降解而不是特定的晶体芯（醇氧化酶蛋白）。

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