首页> 外文期刊>The biochemical journal >Tetraphenylphosphonium ion is a true indicator of negative plasma-membrane potential in the yeast Rhodotorula glutinis. Experiments under osmotic stress and at low external pH values
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Tetraphenylphosphonium ion is a true indicator of negative plasma-membrane potential in the yeast Rhodotorula glutinis. Experiments under osmotic stress and at low external pH values

机译:四苯基膦鏻离子是酵母肾盂肾盂蛋白胶质糖尿中的阴性血浆膜势的真正指标。在渗透压和低外pH值下的实验

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pIn the yeast Rhodotorula glutinis, accumulation of the tetraphenylphosphonium ion (TPP+) was increased under conditions of osmotic stress, indicating a hyperpolarization of the negative membrane potential (delta psi). The following observations were consistent with the occurrence of hyperpolarized delta psi: enhanced accumulation of glucosamine, the uptake of which is also driven by delta psi; increased respiratory rate. The accumulation of TPP+ was gradually decreased by lowering the pH of cell suspensions. At pH values below 4.5, no TPP+ was taken up, but instead thiocyanate (SCN-) was accumulated, indicating a positive delta psi. The pH-dependent influx of glucosamine followed the pattern of TPP+ accumulation both in the wild-type and in the nystatin-resistant mutant, M67, which displayed a negative delta psi down to pH 3. Thus TPP+ accumulation in Rh. glutinis reflected actual electrical potential difference across the plasma membrane./p
机译:在酵母肾窝气泡谷氨酸中,在渗透胁迫条件下增加四苯基膦酰基离子(TPP +),表明负膜电位的超极化(Delta psi)。以下观察结果与超极化δPSI的发生一致:增强葡萄糖胺的积累,其摄取也由Delta Psi驱动;增加呼吸率。通过降低细胞悬浮液的pH,TPP +的积累逐渐降低。在低于4.5以下的pH值下,没有溶解TPP +,而是累积硫氰酸酯(SCN-),表明呈阳性DELTA PSI。葡萄糖胺的pH依赖性流入术后TPP +积聚在野生型和抗阴蛋白抗性突变体M67中,其显示阴性DELTA PSI至pH3。因此,RH中的TPP +积累。谷氨酸反映了血浆膜上的实际电势差。

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