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首页> 外文期刊>Journal of Virology >Transfection of REP- mycoplasmas with viral single-stranded DNA.
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Transfection of REP- mycoplasmas with viral single-stranded DNA.

机译：用病毒单链DNA转染诸如病毒单链DNA的Rep-mycoplasmas。

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Double-stranded DNA from mycoplasma virus L2 can transfect Acholeplasma laidlawii cells in the presence of polyethylene glycol (T. L. Sladek and J. Maniloff, J. Bacteriol. 155:734-741, 1983). We report here that both single-stranded DNA and double-stranded replicative form DNA, from the single-stranded DNA mycoplasma virus L51, are also infectious in this system. For both DNAs transfection frequencies were in the range of 10(-8) transfectants per DNA molecule and 10(-3) transfectants per CFU. An unexpected finding was that both DNAs could transfect A. laidlawii strain REP-, a variant which is a nonpermissive host for single-stranded DNA mycoplasma viruses due to a block in viral DNA replication (Nowak et al., J. Bacteriol. 127:832-836, 1976). The number of viruses produced by transfected REP- cells was comparable to the number produced by both transfected and infected wild-type cells. Therefore, transfected L51 DNAs are able to bypass the replication block in REP- cells that occurs when these cells are infected by L51 virions.
机译：来自支原体病毒L2的双链DNA可以在聚乙二醇的存在下转染Acholeplasma Laidlawii细胞（T.L.Ladkk和J.Maniloff，J.Bacteriol。155：734-741,1983）。我们在此报告，单链DNA和双链复制形式DNA，来自单链DNA支原体病毒L51也在该系统中传染。对于DNA转染频率的频率在10（-8）转染剂的范围内，每个DNA分子和每CFU的10（-3）转染剂。意外的发现是，两种DNA可以转染A.Laidlawii菌株rep-，一种变体，该变体是由于病毒DNA复制的嵌段（Nowak等，J.Bacteriol。127：127： 832-836,1976）。通过转染的脂肪细胞产生的病毒数量与转染和受感染的野生型细胞产生的数量相当。因此，转染的L51 DNA能够在当这些细胞感染L51病毒粒子感染时绕过的复制块。

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