Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

J Luka; W Siegert; G Klein

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Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

机译：溶解的Epstein-Barr病毒确定的核抗原及其表征作为DNA结合蛋白。

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The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

机译：通过高盐处理，从两种EBV转化的细胞系 - Raji和AW-Ramos的分离核溶解Eptstein-Barr病毒（EBV） - 确定核抗原（EBNA）。通过DNA-纤维素色谱法和51Cr释放补体固定测定研究其DNA结合性质。 EBNA与双链和单链小牛胸腺DNA结合，显示对双链DNA的亲和力较高。从Raji和Aw-Ramos细胞制备的EBNA的DNA结合中没有可检测到的差异。

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《Journal of Virology》 |1977年第1期|共8页
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J Luka; W Siegert; G Klein;
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收录信息美国《科学引文索引》(SCI);
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1. Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein. [J] . J Luka, W Siegert, G Klein Journal of Virology . 1977,第1期

机译：溶解的Epstein-Barr病毒确定的核抗原及其表征作为DNA结合蛋白。
2. Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein. [J] . J Luka, W Siegert, G Klein Journal of Virology . 1977,第1期

机译：溶解的Epstein-Barr病毒确定的核抗原及其表征作为DNA结合蛋白。
3. Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit. [J] . J Luka, H J?rnvall, G Klein Journal of Virology . 1980,第3期

机译：Epstein-Barr病毒确定的核抗原和53,000 dalton亚基的相关蛋白质的纯化和生物化学表征。
4. Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins [C] . Lielian Zuo, Meijuan Zhu, Shujuan Du, International Conference on Industrial Technology and Management Science . 2015

机译：Epstein-Barr病毒核抗原1和宿主蛋白相互作用检测的双分子互补系统的建立
5. Purification and Characterization of a Kaposi's Sarcoma-associated Herpesvirus Latency-associated Nuclear Antigen DNA-binding Domain Mutant [D] . Mendoza, Carlie Sunga. 2018

机译：卡波西氏肉瘤相关疱疹病毒潜伏期相关的核抗原DNA结合域突变体的纯化和表征。
6. Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein. [O] . J Luka, W Siegert, G Klein 1977

机译：EB病毒确定的核抗原的增溶及其表征为DNA结合蛋白。
7. Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit [O] . J Luka, H Jörnvall, G Klein 1980

机译：Epstein-Barr病毒确定的核抗原和53,000 dalton亚基的相关蛋白质的纯化和生化表征

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

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