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首页> 外文期刊>Journal of Virology >Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus.
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Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus.

机译：进一步表征呼吸合胞病毒的互补组温度敏感突变体。

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ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was compared with members of the other complementation groups of respiratory syncytial virus (group A [ts-1] and group C [ts-7]). ts-2 was found to be 10- to 1,000-fold more restricted in growth and ability to spread at restrictive temperatures (37, 38, and 39 degrees C) than at the permissive temperature (32 degrees C). In temperature shift-up experiments, the ts defect of ts-1 and other members of complementation group A was found to effect a late function that was required for at least 13 h in the replicative cycle. The ts lesion of ts-7 affected a function early in the replication cycle. In contrast, ts-2 was not temperature sensitive when studied by the shift-up technique. The discrepancy between the ts plaque property and failure to detect temperature sensitivity during the shift-up experiment was resolved when it was shown that ts-2 had a defect in adsorption or penetration or both at the restrictive temperature. Clonal analysis of revertant ts-2 showed a coordinate restoration of ts+ phenotype ans syncytium-forming capacity. It appears that ts-2 has a defect in a protein that is involved in adsorption and/or penetration of virus and is also responsible for cell fusion activity.
机译：与呼吸合胞病毒的其他辅作组的成员进行呼吸合胞病毒和辅酶B组的呼吸合胞病毒和唯一代表的呼吸道合胞病毒和唯一代表的牙菌素形态突变体（A组[TS-1]和C组[TS -7]）。发现TS-2在生长和在限制性温度（37,38和39℃）下散布的能力比在允许温度（32℃）中的增长和延伸的能力为10-至1,000倍。在温度换档实验中，发现TS-1的TS缺陷和互补组A的其他成员在复制循环中实现至少13小时所需的后期功能。 TS-7的TS病变在复制周期早期影响了函数。相反，当通过换档技术研究时，TS-2在温度敏感。当显示TS-2在限制温度下或在限制温度下，在换档实验期间，TS斑块性能和失败检测在换档实验期间失败检测温度敏感性之间的差异。回复剂TS-2的克隆分析显示了TS +表型AS合胞形成能力的坐标恢复。似乎Ts-2在蛋白质中具有缺陷，该蛋白质参与病毒的吸附和/或渗透，并且还负责细胞融合活性。

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