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首页> 外文期刊>Journal of Virology >Effect of interferon on dimethyl sulfoxide-stimulated Friend erythroleukemic cells: ultrastructural and biochemical study.
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Effect of interferon on dimethyl sulfoxide-stimulated Friend erythroleukemic cells: ultrastructural and biochemical study.

机译：干扰素对二甲基亚甲醚刺激的朋友红细胞血症细胞的影响：超微结构和生化研究。

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Treatment of dimethyl sulfoxide-stimulated Friend erythroleukemic cells (clone 745) with mouse interferon (50 U/ml) led to the following changes: (i) a net decrease (40 to 60%) in both the total number of apparently newly synthesized virion particles per cell section and in the average number of cell sections containing one or more virion particles; (ii) a large decrease (80 to 90%) in the number of particles released into the supernatant fluid, as assayed by reverse transcriptase activity; (iii) an initial increase in the number of "immature" or "enveloped A-type" virions followed by an increase in the accumulation of empty, core shell-like particles; and (iv) a decrease in the number of cytoplasmic vacuolar structures, which have been implicated as a major site of virus production and which we show here by serial sectioning to be, in several instances, invaginations of the plasma membrane. The effects on virus production were noticeable 2 h after interferon addition and reached their full extent by 13 h. We conclude from these observations that interferon acts upon the late stage(s) of virion maturation, leading both to a decrease in virion production as well as to the formation of defective particles. In contrast, a small but significant increase in the rate at which globin mRNA and hemoglobin accumulate is observed after interferon treatment.
机译：用小鼠干扰素（50U / mL）处理二甲基亚砜刺激的朋友红细胞血症细胞（克隆745）导致以下变化：（i）显然新合成的病毒群岛的总数净减少（40至60％）每个细胞部分和含有一种或多种病毒粒颗粒的平均细胞部分的颗粒; （ii）通过逆转录酶活性的测定，在释放到上清液中的颗粒的数量的大量减少（80至90％）; （iii）“未成熟”或“包络”或“包络”的初始增加，然后增加空核壳状颗粒的积累; （iv）细胞质染色结构的数量减少，其被牵连作为病毒生产的主要部位，并且我们通过连续切片在此显示在几种情况下，例如血浆膜的侵略性。在干扰素加入后，对病毒产生的影响是显着的2小时，并在13小时内达到全面。我们从这些观察结果中得出结论，干扰素作用于病毒赛成熟的晚期，导致病毒群生产的降低以及形成有缺陷的颗粒。相反，在干扰素治疗后观察到珠蛋白mRNA和血红蛋白积聚的速率的小而显着增加。

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