Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.

C W Clinkscales; M A Bratt; T G Morrison

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Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.

机译：小麦胚芽细胞系统中新城疫病毒多肽的合成。

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We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.

机译：我们从新城疫病毒感染的中国仓鼠卵巢细胞中分离了18秒的RNA，并测试了其在源自小麦胚芽中引导蛋白质合成的能力。该RNA指导的无细胞反应的产品含有从病毒粒中与NP，M，F和47K Rotein煅烧的多肽。此外，产品含有多肽（67K），该多肽（67K）迁移在聚丙烯酰胺凝胶上略微比来自病毒粒子的HN蛋白略微快。从病毒粒子的无细胞产品和蛋白质的胰蛋白酶肽分析证实了它们的身份。

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《Journal of Virology》 |1977年第1期|共5页
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C W Clinkscales; M A Bratt; T G Morrison;
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收录信息美国《科学引文索引》(SCI);
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1. Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system. [J] . C W Clinkscales, M A Bratt, T G Morrison Journal of Virology . 1977,第1期

机译：小麦胚芽细胞系统中新城疫病毒多肽的合成。
2. Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system. [J] . C W Clinkscales, M A Bratt, T G Morrison Journal of Virology . 1977,第1期

机译：小麦胚芽细胞系统中新城疫病毒多肽的合成。
3. Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system. [J] . Shen C, Lee JA, Allam SR Diagnostic microbiology and infectious disease . 2009,第3期

机译：无小麦生殖细胞蛋白质合成系统表达的华支睾吸虫重组抗原的血清学诊断价值。
4. Enhancement of the catalytic activity of the wheat germ, cell-free, protein synthesis system using a fortified translation extract [C] . Young Seoub Park, Hun Su Chu, Cha Yong Choi, Asia-Pacific Chemical Reaction Engineering Symposium(APCRE'05); 20050612-15; Gyeongju(KR) . 2005

机译：使用强化的翻译提取物增强小麦胚芽的无细胞蛋白质合成系统的催化活性
5. Efficacy of a recombinant herpes virus of turkeys vector vaccine, expressing genes to Newcastle disease virus and Marek's disease virus, in chickens and turkeys against exotic Newcastle disease virus challenge. [D] . Heskett, Eric A. 2003

机译：在鸡和火鸡中表达重组新城疫病毒和马立克氏病病毒基因的火鸡载体疫苗重组疱疹病毒抵抗外来新城疫病毒挑战的功效。
6. Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system. [O] . C W Clinkscales, M A Bratt, T G Morrison 1977

机译：无小麦生殖细胞系统中新城疫病毒多肽的合成。
7. Translation of tobacco necrosis virus and its satellite in a cell-free wheat germ system. [O] . Salvato, M S, Fraenkel-Conrat, H 1977

机译：无细胞小麦胚芽系统中烟草坏死病毒及其卫星的翻译。

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.

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