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首页> 外文期刊>Journal of Virology >Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.
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Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.

机译:通过在含抗体琼脂糖凝胶中定量免疫电泳获得的单纯疱疹病毒型1免疫沉淀物的聚丙烯酰胺凝胶电泳分析。

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摘要

Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.
机译:交叉的免疫电泳用于表征由感染的HEP-2细胞产生的疱疹病毒型1(HSV-1)抗原。我们报告了从二维琼脂糖凝胶洗脱的单个抗原抗体沉淀物中的多肽含量分析的方法。分离出HSV-1,Ag-8,Ag-11,Ag-6和Ag-3的四种糖蛋白抗原,并分析多肽含量。提出了多肽的分子量。

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