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首页> 外文期刊>Journal of Virology >Metabolism of uracil-containing DNA: degradation of bacteriophage PBS2 DNA in Bacillus subtilis.
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Metabolism of uracil-containing DNA: degradation of bacteriophage PBS2 DNA in Bacillus subtilis.

机译:含尿嘧啶DNA的代谢:枯草芽孢杆菌中噬菌体PBS2 DNA的降解。

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When Bacillus subtilis is infected by the uracil-containing DNA phage PBS2, the parental DNA labeled with radioactive uracil and cytosine remains acid insoluble. If the synthesis of the phage-induced uracil-DNA N-glycosidase inhibitor is prevented, the parental DNA is completely degraded to acid-soluble products beginning at about 6 min after infection. The host N-glycosidase probably initiates the degradation pathway, with nucleases being responsible for the remaining degradation of the DNA.
机译:当枯草芽孢杆菌被含尿嘧啶的DNA噬菌体PBS2感染时,用放射性尿嘧啶和胞嘧啶标记的亲本DNA保持不溶性。如果预防噬菌体诱导的URACIL-DNA N-糖苷酶抑制剂的合成,则在感染后从约6分钟开始将亲本DNA完全降解到酸溶解产物中。宿主N-糖苷酶可能引发降解途径,核酸酶负责DNA的剩余劣化。

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