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首页> 外文期刊>Journal of Virology >Properties of 'diplophage': a lipid-containing bacteriophage.
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Properties of 'diplophage': a lipid-containing bacteriophage.

机译：“疏透”的性质：含有脂质的噬菌体。

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摘要

We describe the purification and properties of Dp-1, a bacteriophage isolated from Diplococcus pneumoniae. The phage was sensitive to the organic solvents deoxycholate and Sarkosyl, and its infectivity was reduced by treatment with phospholipase C. Electron microscopy indicated the presence of a double-layered coat around the phage particles. Purified phage preparations contained lipid amounting to about 8.5% of the dry weight of the phage, and thin-layer chromatography resolved the lipids into four components. The phage had a buoyant density in CsCl of 1.47 g/cm3, and a sedimentation constant in 0.1 M NaCl of 313S. Analysis in acrylamide gel electrophoresis indicated the presence of three major proteins. Dp-1 DNA shows a density of 1.681 g/cm3. Neutralizing antisera against the phage have a low potency (K less than 120/min).
机译：我们描述了DP-1的纯化和性质，从Diplocococoniae分离的噬菌体。噬菌体对脱氧胆酸盐和甲糖基的有机溶剂敏感，通过用磷脂酶C处理来降低其感染性。电子显微镜表明在噬菌体颗粒周围存在双层涂层。纯化的噬菌体制剂含有脂质的脂质，约8.5％的噬菌体的干重，薄层色谱法将脂质分解成四种组分。噬菌体在1.47g / cm 3的CSCl中具有浮力密度，并且在313s的0.1M NaCl中的沉降常数。丙烯酰胺凝胶电泳分析表明存在三个主要蛋白质。 DP-1 DNA显示密度为1.681g / cm3。反对噬菌体的抗血清的中和抗血清具有低效力（k小于120 / min）。

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