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首页> 外文期刊>Journal of Virology >Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles.
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Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles.

机译:耐链霉素抗性大肠杆菌突变体温度敏感Qbeta - 感染颗粒的产生。

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摘要

A streptomycin-resistant Escherichia coli mutant has been isolated that is temperature sensitive for Qbeta phage, but not for the group I RNA phages f2, MS2, and R17. The growth of Qbeta in the mutant at the nonpermissive temperature (42 degrees C) results in the release of a near-normal burst of noninfectious particles that cosediment with Qbeta in a sucrose gradient. It is assumed that the mutant is defective at elevated temperatures in the suppression of nonsense codons, thereby producing Qbeta-like particles which are noninfectious because of the lack of the read-through protein A1.
机译:已经分离了抗链霉素抗性大肠杆菌突变体,其是对Qβ噬菌体的温度敏感,但不适用于I族RNA噬菌体F2,MS2和R17。在非智力温度(42℃)的突变体中Qbeta的生长导致释放在蔗糖梯度中用Qbeta施用的近似正常爆发。假设突变体在抑制无意义密码子的升高的温度下有缺陷,从而产生Qbeta样颗粒,由于缺乏读取蛋白A1,这是非缺陷的。

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