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首页> 外文期刊>Journal of Virology >Control of protein synthesis in Semliki forest virus-infected cells.
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Control of protein synthesis in Semliki forest virus-infected cells.

机译：Semliki林病毒感染细胞中蛋白质合成的控制。

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Protein synthesis in Semliki forest virus-infected chicken embryo cells was studied by labeling them with [35S]methionine for short periods at different times after infection, with or without synchronization of protein synthesis by the hypertonic block technique. The rate of host-cell protein synthesis declined almost linearly in inverse correlation to the increase in the amount of virus specific RNA. At 5.5 h postinfection, the host-cell protein synthesis was reduced by about 70%. The viral structural proteins were detectable with certainty at 3.5 h postinfection, and their rate of synthesis increased linearly parallel to the amount of their messenger, the 26S RNA. This suggests that the rate of synthesis of the structural proteins is controlled at the level of transcription. The rate of synthesis of the virus-specific nonstructural proteins attained its maximum between 3 and 4 h postinfection and declined thereafter, wheras the amount of their messenger, the 42S RNA, continued to increase linearly in the cells. Thus, the messenger activity of the 42S RNA is reduced in the late phase of infection compared with its activity in the early phase.
机译：通过在感染后的不同时间的短时间将它们与[35s]甲硫氨酸标记，研究了Semliki森林病毒感染鸡胚细胞中的蛋白质合成，用高渗块技术与蛋白质合成的同步，用[35s]甲硫氨酸。宿主细胞蛋白质合成率几乎线性地呈与病毒特异性RNA的增加的增加。在55小时后，宿主细胞蛋白合成减少了约70％。病毒结构蛋白在3.5小时后可检测到3.5小时，它们的合成率随着其信使量的线性平行增加，26s RNA。这表明结构蛋白的合成速率在转录的水平下控制。病毒特异性非结构蛋白的合成速率达到了3至4小时的最大染色，然后下降，其信使的量，42S RNA在细胞中连续增加。因此，与早期阶段的活性相比，42S RNA的信使活性降低了感染后期。

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