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首页> 外文期刊>Journal of Virology >Virus-secific transcription in 3T3 cells transformed by the ts-a mutant of polyoma virus.
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Virus-secific transcription in 3T3 cells transformed by the ts-a mutant of polyoma virus.

机译:由TS-A突变体转化的3T3细胞中的病毒 - SIFIC转录。

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摘要

Virus-specific RNA transcription has been measured in 3T3 cells transformed by the ts-a mutant of polyoma virus by RNA-excess hybridization to the separated strands of polyoma DNA. In two cloned sublines maintained at 39 degrees C, the nonpermissive temperature for the A gene function, RNA transcripts of a large fraction of the "early" strand are detected in both nuclear and cytoplasmic RNA fractions, but no "late" strand transcription is detected. Temperature shift to 31.5 degrees C, the permissive temperature, induces viral DNA replication and virus production accompanied by late strand transcription. In two independently derived noninducible cell lines, L strand transcription is never observed, even after cultivation at the permissive temperature. A smaller fraction of the E strand is transcribed in each noninducible cell than in its inducible parent, and this difference is further characterized as a lack of transcripts of portions of HpaII restriction endonuclease fragments 2 and 6.
机译:已经在由TS-A-A突变体通过RNA过量的杂交转化为PolyoMa DNA的分离链的3T3细胞中测量了病毒特异性RNA转录。在两个克隆的副在39摄氏度保持在39摄氏度中,在核和细胞质RNA级分中检测到基因函数的非智能温度,大部分“早期”链的RNA转录物,但未检测到“晚期”链转录。温度变化至31.5摄氏度,允许温度,诱导病毒DNA复制和病毒产生伴有晚期股线转录。在两个独立衍生的非廉布细胞系中,即使在允许温度培养后,也从未观察到L链转录。较小的E股线在每个不挤出的细胞中转录而不是其诱导母体,并且这种差异进一步表征为HPAII限制性内切核酸酶片段2和6的部分缺乏转录物。

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  • 来源
    《Journal of Virology》 |1977年第1期|共11页
  • 作者

    L T Bacheler;

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  • 收录信息 美国《科学引文索引》(SCI);
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