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首页> 外文期刊>Journal of Virology >SP-10 bacteriophage-specific nucleic acid and enzyme synthesis in Bacillus subtilis W23.
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SP-10 bacteriophage-specific nucleic acid and enzyme synthesis in Bacillus subtilis W23.

机译：SP-10枯草芽孢杆菌W23中的SP-10噬菌体特异性核酸和酶合成。

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Bacillus subtilis W23 was infected with a clear-plaque variant of SP-10 phage, namely, SP-10c. Exogenous thymidine was not incorporated into phage DNA (even in the presence of deoxyadenosine), nor was there any transfer of thymidine nucleotides from bacterial to viral DNA. The lytic program was unaffected by concentrations of 5-fluorodeoxyuridine sufficient to reduce bacterial DNA synthesis by greater than 95%. Although these data are consistent with the interpretation that thymidine nucleotides are excluded from phage DNA, formic acid digests of SP-10c DNA contained what appeared to be the four conventional bases; however, adenine and thymine were not recovered in equimolar yields. DNA-RNA hybridization and hybridization competition experiments were done. Synthesis of host RNA started to wane moments postinfection and stopped completely by 36 min. SP-10c coded for discrete classes of early and late RNA. The possibility of discrete subclasses of early RNA exists. Replication of the bacterial genome appeared to terminate 12 min postinfection. Degradation of the host DNA to acid-soluble material started at 36 min and, by the end of the latent period, greater than 90% of the host chromosome was hydrolyzed. Four apparent phage-coded enzymes have been identified. A di- and triphosphatase degraded dUTP, dUDP, dTTP, and dTDP (and, to a lesser extent, dCDP and d CTP) to the corresponding monophosphates; the enzyme had no apparent activity on dATP and dGTP. SP10c also coded for a DNA-dependent DNA polymerase, lysozyme, and a nuclease that degrades native bacterial DNA. Judging from the dependence of enzyme synthesis on the time of addition of rifampin (an inhibitor of the initiation of RNA synthesis), messengers for the di- and triphosphatase, as well as the nuclease, are transcribed from promoters that start to function 6 min postinfection. Promoters for polymerase and lysozyme did not become functional until 8 and 16 min postinfection, respectively.
机译：枯草芽孢杆菌W23被SP-10噬菌体的透明斑块变体感染，即SP-10C。外源性胸苷未掺入噬菌体DNA中（即使在脱氧腺苷存在下），也没有将胸苷核苷酸与细菌转移到病毒DNA中。裂解程序不受5-氟脱氧核素浓度的影响，足以减少细菌DNA合成大于95％。尽管这些数据与解释一致，但是胸苷核苷酸被排除在噬菌体DNA之外，SP-10C DNA的甲酸消化物包含出现的是四种常规碱;然而，腺嘌呤和胸腺嘧啶未以等摩尔产率恢复。 DNA-RNA杂交和杂交竞争实验已经完成。宿主RNA的合成开始衰竭后甜点，并完全停止36分钟。 SP-10C编码，用于离散的早期和晚期RNA。存在离散RNA的离散亚类的可能性。细菌基因组的复制似乎终止12分钟。将宿主DNA降解到酸溶材料的溶解材料在36分钟开始，并且在潜伏的时期结束时，大于90％的宿主染色体被水解。已经鉴定了四种表观噬菌体编码酶。将DI-和三磷酸酶降解了DUTP，DUDP，DTTP和DTDP（以及较小程度，DCDP和D CTP）的相应单磷酸盐;酶对DATP和DGTP没有明显的活性。 SP10C还编码用于DNA依赖性DNA聚合酶，溶菌酶和降解天然细菌DNA的核酸酶。从酶合成的依赖性判断在加入利福平（RNA合成的引发的抑制剂）中，用于二磷酸酶以及核酸酶的信使，以及核酸酶，从开始起作用6分钟的促进剂的启动子转录。聚合酶和溶菌酶的启动子分别在8和16分钟的染色中均未变成功能性。

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