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首页> 外文期刊>Journal of Virology >Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen.
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Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen.

机译：影响禽肉瘤病毒B77菌株B77菌株的宿主范围的自发突变涉及Vior封套抗原的特异性变化。

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摘要

Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II, mutates either to virus that efficiently infects duck cells (B77 virus-III) or to virus that does not mutate to the ability to infect duck cells (B77 virus-I) (Zarling and Temin, 1976). No significant differences in either the virion envelope glycoproteins or other major virion proteins were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, pseudotypes of B77 virus-I with proteins of a transformation-defective mutant of B77 virus-III formed foci efficiently in duck cells. An alteration in the envelope protein of B77 virus-I was demonstrated by experiments in which B77 firus-I was fused into duck cells with UV-irradiated Sendai virus and formed foci. Neutralization experiments further demonstrated that B77 virus host-range mutants have altered type-specific envelope antigens. Thus, the spontaneous mutations in the host-range gene of B77 virus involve changes in the type-specific virion envelope antigen.
机译：以前表明，Bratislava病毒（B77病毒）的Bratislava菌株的宿主基因以非常高的速率自发突变。伴有B77病毒-II的野生型B77病毒，对病毒有效地感染鸭细胞（B77病毒-III）或不突变感染鸭细胞的病毒（B77病毒-I）（Zarling和Temin，1976）。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳检测病毒素封套糖蛋白或其他主要病毒蛋白蛋白没有显着差异。然而，B77病毒-I的假型与B77病毒-III的转化缺陷突变体的蛋白质有效地在鸭细胞中形成焦点。通过实验证明了B77病毒-I的包络蛋白的改变，其中B77 Furus-i与紫外线辐照的仙台病毒和形成焦点融合成鸭细胞。中和实验进一步证明B77病毒宿主范围突变体具有改变的型特异性包膜抗原。因此，B77病毒的宿主范围基因中的自发突变涉及类型特异性病毒藻囊络抗原的变化。

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