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首页> 外文期刊>Journal of Virology >Flow field-flow fractionation: new method for separating, purifying, and characterizing the diffusivity of viruses.
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Flow field-flow fractionation: new method for separating, purifying, and characterizing the diffusivity of viruses.

机译：流场 - 流分馏：用于分离，纯化和表征病毒扩散性的新方法。

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The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However, a mild adsorption effect is indicated and requires the development of alternate strategies for measuring diffusion coefficients. For Qbeta, our value agrees well within 10% of literature values; the values obtained for other viruses, using Abeta as an internal standard, are untested. Finally, it is demonstrated that flow field-flow fractionation can cleanly fractionate two viruses from one another and from an albumin impurity, that samples as large as several milligrams in size can be analyzed, and that the method has potential utility in the quantitative and qualitative analysis of virus systems.
机译：描述了流场流动分馏的性质和理论，讨论了其对病毒样颗粒的潜在适用性。显示不同的病毒类型将保留在不同的级别。可以通过实验参数的变化来控制保留，与理论吻合良好。然而，指示了一种温和的吸附效果，并且需要开发用于测量扩散系数的交替策略。对于Qbeta，我们的价值在文学价值的10％内讨论;使用ABETA作为内标的其他病毒获得的值未经测试。最后，证明流动场流量分馏可以彼此清洁两种病毒，并且可以分析大小为几毫克的样品，并且该方法具有定量和定性的潜在效用病毒系统分析。

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