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首页> 外文期刊>Journal of Virology >Rescue and Transmission of a Replication-Defective Variant of Moloney Murine Leukemia Virus
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Rescue and Transmission of a Replication-Defective Variant of Moloney Murine Leukemia Virus

机译：莫尼鼠白血病病毒复制缺陷变种的拯救和传输

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We have described a clone of mouse cells, termed “8A,” which appears to be infected with a replication-defective variant of Moloney murine leukemia virus (MuLV) (Rein et al., J. Virol. >25:146-156, 1978). Clone 8A cells release virus particles which do not form plaques in the standard XC test. However, approximately 102 particles per ml of clone 8A supernatant do form plaques in a modified XC test (the “complementation plaque assay”), in which the assay cells are coinfected with the XC-negative, nondefective amphotropic MuLV as well as the test virus. Superinfection of clone 8A cells themselves with amphotropic MuLV results in the production of ～105, rather than ～102, particles per ml which register in the complementation plaque assay. This increase is due to the rescue of replication-defective ecotropic MuLV from clone 8A cells by amphotropic MuLV since (i) this ecotropic MuLV can only form XC plaques in cells which are coinfected with amphotropic MuLV; and (ii) it is possible to transmit this defective variant, rescued from superinfected clone 8A cells, to a fresh clone of normal mouse cells. The time course of production of the rescued MuLV particles by superinfected clone 8A cells is virtually identical to that of rescue from these cells of murine sarcoma virus. Amphotropic MuLV superinfection of “NP-N” cells, which contain a “non-plaque-forming” variant of N-tropic MuLV (Hopkins and Jolicoeur, J. Virol. >16:991-999, 1975), also increases the titer of particles registering in the complementation plaque assay; thus, NP-N cells, like clone 8A cells, contain a rescuable defective variant of ecotropic MuLV.
机译：我们已经描述了一种小鼠细胞的克隆，称为“8a”，其似乎感染莫尼鼠白血病病毒（Mulv）的复制缺陷变体（Rein等，J.Virol。> 25 ：146-156,1978）。克隆8A细胞释放病毒颗粒，该病毒颗粒在标准XC试验中不形成斑块。然而，每毫升克隆8A上清液中的约10 2颗粒在改性的XC试验中形成斑块（“互补斑块测定”），其中测定细胞用XC阴性，非裸射液互化amphotropic Mulv以及测试病毒。克隆8A细胞本身具有含视聚摩尔的SuperFection，导致生产〜10 5 ，而不是〜10 2 ，每mL颗粒在互补斑块测定中寄存在互补斑块测定中。这种增加是由于（i）从克隆8a细胞拯救了来自克隆8a细胞的复制缺陷的生态蛋白，因此这种生态覆盖物可以仅在用两光粒草莫夫繁殖的细胞中形成Xc斑块; （ii）可以将该缺陷的变体传递给从过度的克隆8a细胞振荡到正常小鼠细胞的新鲜克隆中。通过超素克隆8A细胞产生救出的Mulv颗粒的时间过程几乎与来自鼠肉瘤病毒的这些细胞的救援的时间相同。含有“NP-N”细胞的镜片Mulv Superfection，其含有N-Tropic Mulv（Hopkins和Jolicoeur，J.Virol的非斑块成形“变体。> 16 ：991-999，1975 ），还增加了在互补斑块测定中注册的粒子的滴度;因此，如克隆8A细胞，NP-N细胞含有救援造型的生态学Mulv的缺陷变体。

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