• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Effect of 5-methylcytidine on virus production in avian sarcoma virus-infected chicken embryo cells.
【24h】

Effect of 5-methylcytidine on virus production in avian sarcoma virus-infected chicken embryo cells.

机译：5-甲基胞苷对禽类病毒感染鸡胚细胞病毒生成的影响。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

5-Methylcytidine (5mC) is a minor constituent of RNA in procaryotes as well as eucaryotes. The function of this modified nucleoside is not known. We studied the effect of this compound on virus production in avian sarcoma virus-infected chicken embryo fibroblasts. We found, surprisingly, that virus release into the medium was severely reduced in cultures treated with 5mC. In contrast to the effect of 5mC on virus release, intracellular levels of virus-specific RNA transcripts as well as the proteins were slightly elevated. Analysis of intracellular RNA transcripts on velocity gradients and virus-specific proteins in polyacrylamide gels did not reveal any qualitative differences in 5mC-treated cells compared to cytidine-treated cells. From these results we conclude that the effect of 5mC is probably at the level of virus maturation or packaging.
机译：5-甲基胞嘧啶（5MC）是预rNA中的RNA和纽约州的次要组成部分。该修饰的核苷的功能是未知的。我们研究了这种化合物对禽类病毒感染鸡胚胚胎成纤维细胞病毒生产的影响。令人惊讶的是，我们发现，在用5MC处理的培养物中严重降低了病毒释放到培养基中。与5MC对病毒释放的影响相反，细胞内水平的病毒特异性RNA转录物以及蛋白质略微升高。与胞苷处理的细胞相比，聚丙烯酰胺凝胶中速度梯度对速度梯度和病毒特异性蛋白质的分析不透露5MC处理细胞的任何定性差异。从这些结果来看，我们得出结论，5MC的效果可能处于病毒成熟或包装的水平。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号