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首页> 外文期刊>The biochemical journal >The effect of colchicine on human blood platelets under conditions of short-term incubation
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The effect of colchicine on human blood platelets under conditions of short-term incubation

机译：短期孵育条件下秋水仙碱对人血小板的影响

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pThe effects of colchicine on ADP-induced aggregation and on the phosphorylation of tubulin-like protein from human blood platelets were studied. Colchicine at 2mM concentration completely inhibits ADP-induced aggregation after 8min incubation. Under the same inhibitory conditions, phosphorylation of tubulin-like materials in intact platelets was also impaired whereas the endogenous kinase activity of tubulin, isolated through polymerization–depolymerization cycles, was not affected. It was also shown that, under conditions of maximal inhibition of both aggregation and tubulin phosphorylation, colchicine does not penetrate into the cells. The results obtained suggest that the effect of colchicine on platelet aggregation might be mainly, although not exclusively, due to a non-specific effect of the alkaloid on the plasma membrane, rather than to a direct action of the drug on the microtubular protein subunits./p
机译：研究了秋水仙碱对来自人血小板血小板的ADP诱导的聚集和磷酸蛋白样蛋白的磷酸化。在8min孵育后，2mm浓度在2mm浓度下的血清序列完全抑制ADP诱导的聚集。在相同的抑制条件下，在完整血小板中的微管蛋白样材料的磷酸化也受到损害，而通过聚合 - 解聚循环分离的管蛋白的内源激酶活性不受影响。还表明，在最大抑制聚集和小管蛋白磷酸化的条件下，血清序不会渗透到细胞中。得到的结果表明，由于生物碱对质膜上的非特异性效果，血清曲霉对血小板聚集的影响可能主要是，而不是仅仅是对微量蛋白质亚基对药物的直接作用。

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