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首页> 外文期刊>Journal of Virology >Immunological characterization of mouse mammary tumor virus p10 and its presence in mammary tumors and sera of tumor-bearing mice.
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Immunological characterization of mouse mammary tumor virus p10 and its presence in mammary tumors and sera of tumor-bearing mice.

机译：小鼠乳腺肿瘤病毒P10的免疫学特征及其在乳腺肿瘤中的存在和肿瘤瘤小鼠血清。

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摘要

Mouse mammary tumor virus (MMTV) p10 and gp52 were purified and used as radiolabeled antigens in sensitive radioimmunoassays. These radioimmunoassays were specific for MMTV proteins since detergent-disrupted MMTV from C3H/HeN, RIII, and GR/N mice gave complete competition, whereas C3H/HeNf liver extracts and other lysed retroviruses did not. Both gp52 and p10 are coded by the viral genome, since MMTV grown in a heterologous cell line (feline kidney cells) competed in these assays. Sera from mammary tumor-bearing mice and mammary tumors from C3H/HeN and C3H/HeNf mice competed in both the gp52 and the p10 assays. Although these radioimmunoassays detected predominantly group-specific antigenic determinants in C3H/HeN and C3H/HeNf tumor extracts, type specificity was also found with gp52. Absorption of the anti-MMTV serum with C3H/HeNf tumor extracts removed all antibodies directed against p10 and decreased the anti-gp52 titer approximately 30-fold. When this absorbed antiserum was used at limiting dilution in the gp52 radioimmunoassay, C3H/HeN tumor extracts gave complete competition, whereas no competition was found with C3H/HeNf tumor extracts.
机译：纯化小鼠乳腺肿瘤病毒（MMTV）P10和GP52并用作敏感放射免疫测定中的放射性标记抗原。这些放射免疫测定法对MMTV蛋白特异性，因为来自C3H /母鸡，RIII和GR / N小鼠的洗涤剂破坏了MMTV，而C3H / HENF肝脏提取物和其他裂解的逆转录病毒没有。 GP52和P10都是由病毒基因组编码的，因为在这些测定中竞争的异源细胞系（猫肾细胞）中生长的MMTV。来自乳腺肿瘤的小鼠和来自C3H / HEN和C3H / HENF小鼠的乳腺癌的血清竞争GP52和P10测定。虽然这些放射免疫测定主要在C3H /母鸡和C3H / HENF肿瘤提取物中检测到特异性抗原性决定因素，但也发现了GP52的特异性。用C3H / HENF肿瘤提取物吸收抗MMTV血清，除去针对P10的所有抗体并降低抗GP52滴度约30倍。当在GP52放射免疫测定中限制稀释时使用这种吸收的抗血清时，C3H /母鸡肿瘤提取物得到了完全的竞争，而C3H / HENF肿瘤提取物没有发现竞争。

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