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首页> 外文期刊>Journal of Virology >Impaired Maturation of Mouse Mammary Tumor Virus Precursor Polypeptides in Lymphoid Leukemia Cells, Producing Intracytoplasmic A Particles and No Extracellular B-Type Virions
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Impaired Maturation of Mouse Mammary Tumor Virus Precursor Polypeptides in Lymphoid Leukemia Cells, Producing Intracytoplasmic A Particles and No Extracellular B-Type Virions

机译：小鼠乳腺肿瘤病毒前体多肽在淋巴白血病细胞中的成熟受损，产生卵胞质颗粒，没有细胞外B型病毒粒子

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Processing of polypeptides of the mouse mammary tumor virus, a type B retrovirus, was investigated in a transplanted thymic lymphoma cell line of the GR strain (GRSL). This cell line was maintained in vivo in ascites form and in vitro as a suspension culture. GRSL cells produce clusters of intracytoplasmic A particles and are virtually deficient in the production of mature extracellular B-type particles. As control, a mammary tumor cell line of the same mouse strain capable of complete virion synthesis was used. The kinetics of viral polypeptide synthesis were studied by pulse labeling with various isotopes (including 35S and 32P), followed by immunoprecipitation of cell lysates with monospecific antisera to the major mouse mammary tumor virus gag and env proteins, p27 and gp52, respectively. Both the primary gag and env precursor polypeptides were synthesized in the GRSL cells, but their conversion into viral proteins was impaired. The major gag precursor, Pr73gag, was stable over a period of 8 h, and mature viral core polypeptides could not be detected. Also, the highly phosphorylated intermediates in the proteolytic processing of Pr73gag in virus-producing cells were absent in GRSL cells. By immunoprecipitation, Pr73gag was detected in a GRSL particle fraction with the density of intracytoplasmic A particles. The precursor for envelope proteins, Pr73env, was turned over without the generation of mature viral envelope components gp52 and gp36. The in vivo-transplanted ascites GRSL cells, however, were shown to express gp52 on the cell surface together with a 73,000-dalton polypeptide, as indicated by cell surface iodination and immunoprecipitation.
机译：在GR菌株（GRSL）的移植的胸腺淋巴瘤细胞系中研究了小鼠乳腺肿瘤病毒的多肽，B型逆转录病毒。将该细胞系以腹水形式维持在体内并体外作为悬浮培养。 GRSL细胞产生粒细胞颗粒的簇，并且在成熟细胞外B型颗粒的产生下几乎不足。作为控制，使用能够完全雌激素合成的相同小鼠菌株的乳腺肿瘤细胞系。通过用各种同位素（包括 35℃和 32℃）来研究病毒多肽合成的动力学，然后用单特异性抗血清的细胞裂解物免疫沉淀到主要小鼠乳腺肿瘤病毒 GAG 和 ENV 蛋白，P27和GP52分别。在GRSL细胞中合成初级 GAG 和 ENV 前体多肽，但它们转化为病毒蛋白损害。主要 GAG 前体，PR73 Gag 在8小时的时间内稳定，并且无法检测到成熟的病毒核心多肽。此外，在GRSL细胞中不存在PR73 Gag Gag 的高磷酸化中间体。通过免疫沉淀，在具有卵胞上质密度颗粒的GRSL颗粒部分中检测到PR73 gag 。包膜蛋白质的前体PR73 ENV ，在没有成熟病毒包络组分GP52和GP36的情况下逆转。然而，如细胞表面碘化和免疫沉淀所示，在细胞表面上表达在细胞表面上的GP52，如细胞表面碘化和免疫沉淀所示的体内移植的腹水细胞。

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