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首页> 外文期刊>Journal of Virology >Purification and characterization of an early glycoprotein from adenovirus type 2-infected cells.
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Purification and characterization of an early glycoprotein from adenovirus type 2-infected cells.

机译：腺病毒2型感染细胞早期糖蛋白的纯化和表征。

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摘要

An adenovirus type 2 early glycoprotein with an apparent molecular weight of 19,000 (E19K) in sodium dodecyl sulfate-polyacrylamide gels has been extensively purified. Purification involved detergent solubilization of membrane fractions from infected cells, followed by affinity chromatography on a lectin column and DEAE-Sephadex chromatography. The purified material contained three polypeptides (E40K, E19K, E17.5K), with approximately 90% of the material in the E19K moiety. All three polypeptides yielded identical tryptic peptide maps. The E19K polypeptide contained glucosamine as revealed by [3H]glucosamine labeling of infected cells and amino acid analysis of the purified protein. Immunoprecipitation with a monospecific antiserum showed that the E19K polypeptide started to be synthesized at 2 h, with a maximal rate at 4 h after infection. It was also synthesized at a low rate late in the infectious cycle (12 to 24 h postinfection). Immunoprecipitation from three adenovirus type 2-transformed hamster embryo cell lines and two adenovirus type 2-transformed rat cell lines revealed that one of the hamster cell lines (ad2HE4) and one of the rat cell lines (A2T2C4) expressed this protein.
机译：广泛地纯化了一种腺病毒2型具有表观分子量的早期糖蛋白，具有19,000（E19K）的十二烷基硫酸钠 - 聚丙烯酰胺凝胶。纯化涉及来自感染细胞的膜级分的洗涤剂溶解，然后在凝集素柱和DEAE-Sephadex色谱上进行亲和力色谱。纯化的材料含有三种多肽（E40K，E19K，E17.5K），在E19K部分中具有约90％的材料。所有三种多肽均产生相同的胰蛋白酶肽图。 E19K多肽含有葡萄糖胺，如[3H]葡萄糖胺标记的感染细胞和纯化蛋白质的氨基酸分析。用单特异性抗血清的免疫沉淀表明，E19K多肽在2小时开始合成，感染后4小时以最大速率合成。它也以感染循环晚期的低速（12至24小时）合成的是它。来自三种腺病毒2-转化的仓鼠胚胎细胞系的免疫沉淀和两个腺病毒2型转化大鼠细胞系显示，仓鼠细胞系（AD2HE4）和一种大鼠细胞系（A2T2C4）中的一种表达该蛋白质。

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