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首页> 外文期刊>Journal of Virology >Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma
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Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma

机译：EV-2的特征，一种从欧洲鳗鱼（Anguilla Anguilla）孤立的病毒与气孔缺陷

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A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels (Anguilla anguilla) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm3. EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence.
机译：指定的EV-2已经从外部肿瘤组织和欧洲鳗鱼的内脏（ Anguilla Anguilla ）中分离出来的病毒。它含有RNA，是4℃以上的乙醚，酸和温度不稳定，浓缩制剂凝集鸡肉和绵羊红细胞。在感染的前2.75小时期间添加放线霉素D抑制病毒复制。如蔗糖梯度确定，病毒的浮力密度为1.19g / cm 3 。 EV-2具有适度的亲子性球形形态;它的直径范围为80至140nm。病毒群岛具有窄，定期间隔的表面凸起约10nm。在15°C的FHM细胞中复制显示出现在10小时的新感染性，并在20小时内到达高原。细胞病变效应包括细胞融合，同义性和不规则圆形细胞块。通过特异性免疫荧光在感染细胞的细胞质中检测到病毒抗原。

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