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首页> 外文期刊>Journal of Virology >Induction of thymidine kinase and DNase in varicella-zoster virus-infected cells and kinetic properties of the virus-induced thymidine kinase.
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Induction of thymidine kinase and DNase in varicella-zoster virus-infected cells and kinetic properties of the virus-induced thymidine kinase.

机译：诱导胸苷激酶和DNA酶在种子菌 - 带状病毒感染细胞和病毒诱导的胸苷激酶的动力学性质中的诱导。

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摘要

Thymidine kinase (TK), DNA polymerase, and DNase activities were induced in human foreskin fibroblasts after varicella-zoster virus infection. The induced TK and DNase activities have electrophoretic mobilities different from the corresponding host enzymes. Varicella-zoster virus-induced TK was purified and separated from the host enzyme by affinity column chromatography. This enzyme has been shown to have a broader substrate specificity with respect to either the phosphate donor or acceptor as compared with human cytoplasmic and mitochondrial TKs. The best phosphate donor is ATP, with a Km of 16 microM. The Km values of thymidine, deoxycytidine, and 5-propyl deoxyuridine were estimated to be 0.4, 180, and 0.8 microM, respectively. The Ki values for several analogs of thymidine such as 5-iododeoxyuridine, arabinofuranosylthymine, 5-ethyl deoxyuridine, and 5-cyanodeoxyuridine were also examined. TTP acted as a noncompetitive inhibitor with respect to thymidine with a Ki of 5 microM. The kinetic behavior of varicella-zoster virus-induced TK is different from human cytoplasmic, human mitochondrial, and herpes simplex virus type 1- and 2-induced TKs.
机译：在毒液蛋果蛋白酶感染后，在人包皮成纤维细胞中诱导胸苷激酶（TK），DNA聚合酶和DNase活性。诱导的TK和DNase活性具有与相应的宿主酶不同的电泳迁移率。通过亲和柱色谱法纯化VariCella-Zoster病毒诱导的TK并与宿主酶分离。与人细胞质和线粒体TKS相比，该酶已经显示出相对于磷酸盐供体或受体具有更宽的底物特异性。最佳的磷酸盐供体是ATP，km为16微米。胸苷，脱氧胞苷和5-丙基脱氧尿苷的km值分别估计为0.4,180和0.8微米。还研究了几种胸苷类胸苷，如5-碘氧基脲，阿拉米蛋白酶蛋白，5-乙基脱氧尿苷和5-氰基氧酰亚胺尿苷的Ki值。 TTP相对于胸苷的非竞争性抑制剂，ki为5微米。 VariCella-Zoster病毒诱导的TK的动力学行为不同于人细胞质，人体线粒体和单纯疱疹病毒1-和2诱导的TKS。

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