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首页> 外文期刊>Journal of Virology >Simian virus 40 T- and U-antigens: immunological characterization and localization in different nuclear subfractions of simian virus 40-transformed cells.
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Simian virus 40 T- and U-antigens: immunological characterization and localization in different nuclear subfractions of simian virus 40-transformed cells.

机译：Simian病毒40 T-和U-抗原：Simian病毒40转化细胞不同核偶联中的免疫表征和定位。

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摘要

Simian virus 40 (SV40)-transformed cells and cells infected by the nondefective adenovirus 2(Ad2)-SV40 hybrid viruses Ad2+ND1 and Ad2+ND2 were analyzed for SV40 T- and U-antigens, respectively, using individual hamster SV40 tumor sera or serum for which U-antibodies were removd by absorption. These studies showed that (i) T- and U-antigens can be defined by separate classes of antigenic determinants and (ii) the U-antigenic determinants in SV40-transformed cells and in hybrid virus-infected cells are similar. The apparent discrepancy in the subcellular location of U-antigen in SV40-transformed cells (nuclear location) and in hybrid virus-infected cells (perinuclear location) as determined by immunofluorescence staining of methanol/acetone-fixed cells could be resolved by treating hybrid virus-infected cells with a hypotonic KCl solution before fixation. Upon this treatment hybrid virus-infected cells also showed nuclear U-antigen staining. The possibility of an association of T- and U-antigens with different nuclear subfractions in SV40-transformed cells was investigated. Detergent-cleaned nuclei of SV40-transformed cells were fractionated into nuclear matrices and a DNase-treated, high-salt nuclear extract. Analysis of the nuclear matrices by immunofluorescence microscopy with T+U+ and T+U- hamster SV40 tumor serum revealed that U-antigen remained associated with the nuclear matrices, whereas T-antigen could not be detected in this nuclear subfraction. T-antigen, however, could be immunoprecipitated from nuclear extracts of the SV40-transformed cells.
机译：用单独的仓鼠SV40肿瘤血清分别分别分别分别分别对SV40 T-和U-抗原进行分析由NondeFective腺病毒2（AD2）-SV40杂交病毒Ad2 + Nd1和Ad2 + Nd2进行切割的细胞和细胞的-Trance病毒40（SV40）的细胞和细胞或者通过吸收去除U-抗体的血清。这些研究表明，（i）T-和U-抗原可以通过单独的抗原决定因素和（ii）在SV40转化细胞中的U-抗原决定因素和杂交病毒感染细胞中相似。通过治疗杂种病毒，可以解决通过免疫荧光染色（核定位）和通过免疫荧光染色而确定的甲醇/丙酮固定细胞的杂交病毒感染细胞（杂交病毒感染细胞（Perinualclear定位）中的表观差异。可以通过治疗杂种病毒来解决 - 在固定前用低旋转KCl溶液的摄取细胞。在该处理后，杂种病毒感染细胞也显示核U-抗原染色。研究了在SV40转化细胞中具有不同核偶极细胞的T-和U-抗原关联的可能性。将洗涤剂清洁的SV40转化细胞的细胞核分离成核基质和DNase治疗的高盐核提取物。用T + U +和T + U-仓鼠SV40肿瘤血清通过免疫荧光显微镜进行核基质的分析显示，与核基质相关的U形抗原，而在该核分关中无法检测到T-抗原。然而，T-抗原可以从SV40转化细胞的核提取物免疫沉淀。

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    《Journal of Virology》 |1979年第2期|共11页
  • 作者

    W Deppert;

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  • 收录信息 美国《科学引文索引》(SCI);
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