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首页> 外文期刊>Journal of Virology >Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice.
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Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice.

机译：组织小鼠乳腺肿瘤病毒特异性DNA内源性对BALB / C小鼠。

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We used restriction endonucleases to prepare physical maps of the mouse mammary tumor virus (MMTV)-specific DNA endogenous to the BALB/c mouse strain. The mapping was facilitated by the DNA transfer procedure, using complementary DNAs specific for the whole and for the 3' terminus of MMTV RNA to detect fragments containing viral sequences. The strategies used for the arrangement of fragments into physical maps included sequential digestions with two or three enzymes; preparative isolation of EcoRI fragments containing viral sequences; and comparisons of virus-specific fragments derived from the DNA of several mouse strains. Most of the MMTV-related DNA in the BALB/c genome is organized into two units (II and III) which strongly resemble proviruses acquired upon horizontal infection with milk-borne strains of MMTV and other retroviruses. These units contain approximately 6.0 x 10(6) Mr of apparently uninterrupted viral sequences, they bear redundant sequences totaling at least 700 to 800 base pairs at their termini, and the terminal redundancies include sequences derived from the 3' end of MMTV RNA. Units II and III are closely related in that they share 12 of 14 recognition sites for endonucleases, but cellular sequences flanking units II and III are dissimilar by this criterion. The remainder of the MMTV-related DNA endogenous to BALB/c mice is found in a single subgenomic unit (unit I) with a complexity of ca. 2 x 10(6) Mr; the structure of this unit has not been further defined. These results support the hypotheses that endogenous proviruses have been acquired by infection of germinal tissues with MMTV. The physical maps are also useful for identifying the MMTV genomes endogenous to BALB/c mice in studies of the natural history of mammary tumorigenesis.
机译：我们使用限制性内切核酸酶来制备内源性对BALB / C小鼠菌株的小鼠乳腺肿瘤病毒（MMTV）的物理图。通过DNA转移程序促进了映射，使用针对整体的互补DNA和MMTV RNA的3'末端检测含有病毒序列的片段。用于将碎片排列成物理图的策略包括具有两种或三种酶的连续消化;含有病毒序列的EcoRI片段的制备分离;和衍生自几种小鼠菌株DNA的病毒特异性片段的比较。 BALB / C基因组中的大多数MMTV相关的DNA被组织成两个单位（II和III），其在水平感染后患有MICKE的MMTV和其他逆转录病毒的乳房菌株获得的潜水术。这些单元含有大约6.0×10（6）MR的明显不间断的病毒序列，它们在其终端中承担了总计至少700至800个碱基对的冗余序列，终端冗余包括衍生自MMTV RNA的3'末端的序列。单位II和III密切相关，因为它们共享14个核酸核酸酶识别位点的12个，但细胞序列侧翼单元II和III通过该标准是不同的。与BALB / C小鼠的剩余的MMTV相关DNA内源性在单一的亚基根据CA的亚基金属单元（单元I）中发现。 2 x 10（6）MR;该单元的结构尚未进一步定义。这些结果支持假设内源性潜水术通过用MMTV感染发芽组织而获得。物理图也可用于鉴定内源性对BALB / C小鼠的MMTV基因组在乳腺瘤瘤的自然病史研究中。

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