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首页> 外文期刊>Journal of bacteriology >Presence and synthesis of cholesterol in stable staphylococcal L-forms.
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Presence and synthesis of cholesterol in stable staphylococcal L-forms.

机译：稳定的葡萄球菌L-型胆固醇的存在和合成。

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摘要

The sterol which was present in two strains of a stable staphylococcal L-form was analyzed by gas-liquid chromatography and combined gas-liquid chromatography-mass spectrometry. The retention time of the sterol on gas-liquid chromatography was the same as that of authentic cholesterol. Analysis of the sterol by mass spectrometry showed a molecular ion at an m/e of 386 and the same patterns of major ions above an m/e of 145 as those of authentic cholesterol. As a result, the sterol in staphylococcal L-form was identified as cholesterol. A parent strain and its L-forms were cultured in medium containing [14C]acetate, and the synthesis of cholesterol was examined. In the L-forms, 0.52% of the total lipid radioactivity was found in cholesterol fraction, whereas no significant radioactivity was detected in the cholesterol fraction of the parent strain, indicating that staphylococcal L-forms have acquired the capacity to synthesize cholesterol.
机译：通过气液相色谱和组合气液色谱 - 质谱法分析两种稳定的葡萄球菌L形式的甾醇。甾醇对气液色谱法的保留时间与正宗胆固醇的保留时间与真实的胆固醇相同。质谱分析甾醇的分析显示为386的M / E的分子离子，其主要离子的相同模式为145的M / E，为真正的胆固醇。结果，将酪蛋白L形式的甾醇鉴定为胆固醇。在含有[14℃]乙酸钾的培养基中培养亲本菌株及其L-型，并检查胆固醇的合成。在L-形式中，在胆固醇级分中发现了0.52％的总脂质放射性，而在亲本菌株的胆固醇部分中没有检测到显着的放射性，表明葡萄球菌L--葡萄球菌获得了合成胆固醇的能力。

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