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首页> 外文期刊>Journal of Virology >Epstein-Barr virus DNA is amplified in transformed lymphocytes.
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Epstein-Barr virus DNA is amplified in transformed lymphocytes.

机译：Epstein-Barr病毒DNA在转化的淋巴细胞中扩增。

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Leukocytes isolated from two adult donors who lacked detectable antibodies to antigens associated with Epstein-Barr virus were exposed to an average of 0.02 to 0.1 DNA-containing particles of Epstein-Barr virus per cell and immediately clones in agarose. Within about 30 generations all transformed cell clones contained between 5 and 800 copies of viral DNA per cell. Only 1 in 10(4) to less than 1 in 10(5) of the cells of each clone release virus, and the frequency of release did not correlate with the average number of copies of viral DNA in the cells of each clone. One clone that had an average of five copies of viral DNA per cell was recloned, and the average number of copies in four of six subclones increased 15-to 50-fold while the subclones were being propagated sufficiently to study them. These results indicate that Epstein-Barr virus DNA can undergo amplification relative to cell DNA at different times after it transforms cells.
机译：从两种成人供体中分离的白细胞被缺乏与抗原抗原的可检测的抗体暴露于每种细胞的平均Epstein-Barr病毒的含Epstein-Barr病毒的0.02至0.1个DNA颗粒，并立即在琼脂糖中克隆。在大约30多种几代内，所有转化的细胞克隆包含在每种细胞5到800拷贝的病毒DNA之间。每种克隆释放病毒的10（5）个细胞中仅为10（4）次以入，释放频率与每个克隆的细胞中病毒DNA的平均拷贝数不相关。每个细胞平均有五个病毒DNA拷贝的一个克隆均升高，并且四个六个亚克酮中的四个拷贝数增加了15％至50倍，而亚克隆正在足够繁殖以研究它们。这些结果表明，在其转化细胞后，Epstein-BART病毒DNA可以在不同时间在不同时间进行扩增。

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