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首页> 外文期刊>Journal of Virology >Herpes Simplex Virus Type 1 Infection of Isogenic Epstein-Barr Virus Genome-Negative and -Positive Burkitt's Lymphoma-Derived Cell Lines
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Herpes Simplex Virus Type 1 Infection of Isogenic Epstein-Barr Virus Genome-Negative and -Positive Burkitt's Lymphoma-Derived Cell Lines

机译：单纯疱疹病毒1型感染中源性肺斯坦 - 巴克病毒基因组阴性和阳性Burkitt的淋巴瘤衍生细胞系

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The Epstein-Barr virus (EBV) genome-negative Burkitt's lymphoma-derived cell lines BJAB and Ramos and their in vitro EBV-converted sublines BJAB-B1, BJAB-A5, BJAB-B95-8, and AW-Ramos were infected with high multiplicities of herpes simplex virus type 1 (HSV-1; 10 to 70 PFU/cell). Cultures were monitored for cell growth and HSV-1 DNA synthesis. EBV-converted BJAB cultures were more permissive for HSV-1 infection than BJAB cultures. Significant cell killing and HSV-1 DNA synthesis were observed during the first 48 h of infection in the EBV-converted BJAB cultures but not in the BJAB cultures. The EBV-converted BJAB-B1 cell line contains an appreciable fraction of EBV-negative cells. Therefore, it was cloned. EBV-positive and -negative cells were identified by using EBV-determined nuclear antigen anti-complement immunofluorescence. Two types of subclones were identified: (i) those which contained both EBV-determined nuclear antigen-positive and -negative cells and (ii) those which contained only EBV-determined nuclear antigen-negative cells. When levels of HSV-1 DNA synthesis were measured in these subclones, it was found that the former were more permissive for HSV-1 infection than the latter. Thus, the presence of the EBV genome in BJAB cells correlates with increased permissiveness of these cells for HSV-1 during the first 48 h of infection. Nonetheless, persistent HSV-1 infections were established in both BJAB and EBV-converted BJAB-B1 cultures. No differences in extent of permissiveness for HSV-1 infection were found for Ramos and EBV-converted AW-Ramos cells.
机译：Epstein-Barr病毒（EBV）基因组阴性Burkitt的淋巴瘤衍生的细胞系Bjab和ramos及其体外EBV转换的载有力Bjab-B1，Bjab-A5，Bjab-B95-8和Aw-Ramos被高分感染单纯疱疹病毒类型1（HSV-1; 10至70 PFU / CELL）的多重性。监测细胞生长和HSV-1 DNA合成的培养物。 EBV转化的BJAB培养物比BJAB培养物更允许HSV-1感染。在EBV转化的BJAB培养物中的前48小时内观察到显着细胞杀伤和HSV-1 DNA合成，但不在BJAB培养物中。 EBV转化的BJAB-B1细胞系含有可观的EBV阴性细胞。因此，它被克隆了。通过使用EBV确定的核抗原抗补体免疫荧光来鉴定EBV-阳性和阴茎。鉴定了两种类型的亚克酮：（i）那些含有EBV确定的核抗原阳性和阴性细胞的那些，并且（ii）仅包含EBV确定的核抗原阴性细胞的那些。当在这些亚克酮中测量HSV-1 DNA合成的水平时，发现前者对HSV-1感染的允许比后者更允许。因此，BJAB细胞中EBV基因组的存在与在感染的前48小时内与HSV-1增加这些细胞的允许增加。尽管如此，在BJAB和EBV转化的BJAB-B1培养物中建立了持续的HSV-1感染。发现RAMOS和EBV转化的AW-RAMOS细胞没有对HSV-1感染的允许允许的允许程度的差异。

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