• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic acids research >Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313
【24h】

Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313

机译：在PBR313中表征四环抗性基因的野生型和突变促进剂

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By employing a system of RNA polymerase binding and restriction endonuclease digestion, we demonstrate that the region in and around the Hind III site of pBR3l3 and pBR322 is the promoter for the tetracycline (Tc) resistance gene(s). Furthermore, it is shown that this region was transferred intact from pSC101 during the construction of the latter plasmids. The in vitro insertion of a few base pairs at the Hind III site produces a series of “down” promoter mutations in which the level of in vivo Tc resistance is reduced. Sequence analysis of the various promoter mutations revealed significant base pair rearrangements in the region between ?40 and ?12 of the promoter. While these base alterations do not appear to affect the firm binding of RNA polymerase, they do affect the ability of mutant promoters to initiate transcription. These observations suggest that the region from ?40 to ?12, previously designated as the “recognition region”, is actually involved in the process of initiation of transcription.
机译：通过使用RNA聚合酶结合和限制性内切核酸酶消化的系统，我们证明了PBR3L3和PBR322的后III位点和周围的区域是用于四环素（TC）抗性基因的启动子。此外，示出在后一种质粒的构建期间从PSC101中完整地转移该区域。在后III位点处的少数碱基对的体外插入产生一系列“向下”启动子突变，其中体内TC电阻的水平降低。各种启动子突变的序列分析显示出在启动子的40和α12之间的区域中的显着碱基对重排。虽然这些基础改变似乎不会影响RNA聚合酶的坚定结合，但它们会影响突变促进剂引发转录的能力。这些观察结果表明，来自α40至12的区域，先前被指定为“识别区域”，实际上是转录的启动过程中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号