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首页> 外文期刊>Journal of Virology >Epstein-Barr Virus-Specific RNA III. Mapping of DNA Encoding Viral RNA in Restringent Infection
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Epstein-Barr Virus-Specific RNA III. Mapping of DNA Encoding Viral RNA in Restringent Infection

机译：Epstein-Barr病毒特异性RNA III。编码病毒RNA在restercent感染中的DNA映射

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Namalwa and Raji cells, originally obtained from a Burkitt tumor biopsy, grow as continuous cell lines in vitro and contain the Epstein-Barr virus (EBV)-related nuclear antigen EBNA (B. M. Reedman and G. Klein, Int. J. Cancer >11:499-520, 1973) and RNA homologous to at least 17 and 30% of the EBV genome, respectively (S. D. Hayward and E. Kieff, J. Virol. >18:518-525, 1976; T. Orellana and E. Kieff, J. Virol. >22:321-330, 1977). The polyribosomal and polyadenylated [poly(A)+] RNA fractions of Namalwa and Raji cells are enriched for a class of viral RNA homologous to 5 to 7% of EBV DNA (Hayward and Kieff, J. Virol. >18:518-525, 1976; Orellana and Kieff, J. Virol. >22:321-330, 1977). The objective of the experiments described in this communication was to determine the location within the map of the EBV genome (D. Given and E. Kieff, J. Virol. >28:524-542, 1978) of the DNA which encodes the viral RNA in the poly(A)+ and non-polyadenylated [poly(A)?] RNA fractions of Namalwa cells. Hybridization of labeled DNA homologous to Namalwa poly(A)+ or poly(A)? RNA to blots containing EcoRI, Hsu I, or Hsu I/EcoRI double-cut fragments of EBV (B95-8) or (W91) DNA indicated that these RNAs are encoded by DNA contained primarily in the Hsu I A/EcoRI A and Hsu I B/EcoRI A fragments and, to a lesser extent, in other fragments of the EBV genome. Hybridizations of Namalwa poly(A)+ and poly(A)? RNA in solution to denatured labeled EcoRI A or B fragments, Hsu I A, B, or D fragments, and Hsu I A/EcoRI A or Bam I S fragments and of Raji polyribosomal poly(A)+ RNA to the EcoRI A fragment indicated that (i) Namalwa poly(A)+ RNA is encoded primarily by 6 × 105 daltons of a 2 × 106-dalton segment of DNA, Bam I S, which is tandemly reiterated, approximately 10 times, in the Hsu I A/EcoRI A fragment and is encoded to a lesser extent by DNA in the Hsu I B, EcoRI B, and Hsu I D fragments. Raji polyribosomal poly(A)+ RNA is encoded by a similar fraction of the EcoRI A fragment as that which encodes Namalwa poly(A)+ RNA. (ii) The fraction of the Bam I S fragment homologous to Namalwa poly(A)? RNA is similar to the fraction homologous to Namalwa poly(A)+ RNA. However, Namalwa poly(A)? RNA is homologous to a larger fraction of the DNA in the Hsu I B, Hsu I D, and EcoRI B fragments.
机译：Namalwa和Raji细胞最初从Burkitt肿瘤活组织检查中获得，体外生长为连续细胞系，含有Epstein-Barr病毒（EBV）的核抗原EBNA（BM Reedman和int。J.Cernams <强> 11 ：499-520,1973）和RNA分别与eBV基因组的至少17和30％同源（SD Hayward和E.Kieff，J.Virol。> 18 ： 518-525,1976; T. Orellana和E.Kieff，J.Virol。> 22 ：321-330,1977）。 Namalwa和Raji细胞的多血糖和聚酰胺化[聚（a）+] RNA部分富集为一类同源的病毒RNA至5至7％的EBV DNA（Hayward和Kieff，J.Virol。> 18 ：518-525，1976;奥雷拉纳和Kieff，J.Virol。> 22 ：321-330,1977）。该通信中描述的实验的目的是确定EBV基因组的地图中的位置（D.给定和E.Kieff，J.Vireol。> 28 ：524-542,1978）编码聚（a）+和非聚腺苷酸化的病毒RNA的DNA和Namalwa细胞的RNA级分。与Namalwa聚（a）+或聚（a）同源标记的DNA杂交？ RNA包含 ECO RI， HSU I，或 HSU I / ECO RI双切割碎片的EBV （B95-8）或（W91）DNA表明，这些RNA由主要包含在 HsU IA / ECO RI A和 HSU IB / ECO RI一个片段，在较小程度上，在EBV基因组的其他片段中。 Namalwa poly（a）+和聚（a）的杂交？溶液中的RNA使标记为 Eco Ri A或B片段， HsU Ia，B或D片段，以及 Hsu IA / ECO RI A或 BAM 是碎片和Raji多标体聚（a）+ RNA至 eco RI a片段表明（i）namalwa poly（a ）+ RNA主要由6×10 5 D室内编码2×10 -dAlton -dalton段的DNA， Bam 是Tandemly重复，大约10次，在 HSU IA / ECO RI一个片段中，并在 Hsu IB中编码到较小程度上， ECO RI B，以及 HSU ID片段。 Raji多丝体聚（a）+ RNA通过类似的零件的类似部分进行编码，作为编码Namalwa poly（a）+ RNA的片段。 （ii）与Namalwa Poly（A）同源的 BAM I S片段的分数？ RNA类似于与Namalwa聚（A）+ RNA同源的级分。但是，namalwa poly（a）？ RNA与 Hsu I B， Hsu I D和 Eco Ri B片段中的较大部分与DNA的较大部分同源。

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