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首页> 外文期刊>Journal of Virology >Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties.
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Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties.

机译：噬菌体T5 I的中断缺陷突变体。隔离和一般性质。

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摘要

Mutations of bacteriophage T5 were isolated which lack one or more of the natural single-chain interruptions that occur in the mature DNA of this virus. Interruption-deficient mutants were detected by screening survivors of hydroxylamine mutagenesis for altered DNA structure by electrophoresis in agarose slab gels. Over 60 independent mutants were isolated from a survey of approximately 800 phages particles. All of the mutants were viable and could be grouped into two classes. Mutants in one class lacked one of the localized sites where interruptions occur in T5 DNA. To date, mutants that affect five different sites have been obtained. Mutants in the other class were essentially free from interruptions or had a reduced frequency of interruptions throughout the genome. The members of this class included several amber mutants. Complementation tests indicated that at least two genes are required for the presence of interruptions in mature T5 DNA.
机译：分离噬菌体T5的突变，其缺乏在该病毒成熟DNA中发生的一种或多种的天然单链中断。通过筛选羟胺诱变的幸存者通过琼脂糖板凝胶中的电泳筛选羟胺诱变的幸存者来检测中断缺陷突变体。从约800个噬菌体颗粒的调查中分离出超过60个独立突变体。所有突变体都是可行的，可以分为两类。一类中的突变体缺少了在T5 DNA中发生中断的局部位点之一。迄今为止，已经获得了影响五个不同网站的突变体。其他类中的突变体基本上没有中断或在整个基因组中具有降低的中断频率。本课程的成员包括几个琥珀色突变体。互补试验表明，成熟T5 DNA中断需要至少两个基因。

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