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首页> 外文期刊>Nucleic acids research >Evidence for the role of double-helical structures in the maturation of Simian Virus-40 messenger RNA
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Evidence for the role of double-helical structures in the maturation of Simian Virus-40 messenger RNA

机译：双螺旋结构在Simian病毒-40信使RNA成熟中作用的证据

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Simian Virus-40 infected BSC-1 cells were pretreated with glucosamine and briefly pulsed with [3H]-uridine. The labeling can be halted instantaneously by the addition of cold uridine and glucosamine. Under these pulse-chase conditions, the inhibitory effects of the intercalating agent proflavine on the processing of prelabeled nuclear RNA precursors were examined in vivo. Proflavine inhibits the cleavage of viral nuclear RNA precursors. However, turnover of the mature viral mRNAs in the cytoplasm is not inhibited. The effect of proflavine on processing is not a secondary consequence of its inhibition of protein synthesis. The data suggest that base-paired secondary structures in the primary transcripts are important processing signals in the generation of viral mRNA molecules.
机译：用葡糖胺预处理Simian病毒-40感染的BSC-1细胞，并用[ 3 h] - 瓜汀简单地脉冲。通过加入冷尿苷和葡糖胺，可以瞬间停止标记。在这些脉冲追踪条件下，在体内检查插层嵌入剂PREMLAVIN对预先标记的核RNA前体的加工的影响。 ProFlavine抑制病毒核RNA前体的切割。然而，不抑制细胞质中成熟病毒MRNA的营业额。预流量对加工的影响不是其抑制蛋白质合成的次要后果。数据表明，初级转录物中的基础配对二次结构是生成病毒mRNA分子中的重要处理信号。

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