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首页> 外文期刊>Journal of Virology >Characterization of tau antigens isolated from uninfected and simian virus 40-infected monkey cells and papovavirus-transformed cells.
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Characterization of tau antigens isolated from uninfected and simian virus 40-infected monkey cells and papovavirus-transformed cells.

机译：从未感染和猿猴病毒40感染猴细胞和乳头病毒转化细胞中分离的Tau抗原的表征。

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摘要

Tau antigens (also known as cellular or nonviral tumor antigens) were detected in uninfected and simian virus 40-infected monkey cells after immunoprecipitation with serum from hamsters bearing simian virus 40-induced tumours (anti-T serum). These two proteins (56,000 daltons) were digested to similarly sized peptides with various amounts of Staphylococcus aureus V8 protease. The Tau antigen isolated from infected monkey cells was closely related but was not identical to the corresponding protein from human cells transformed by simian virus 40, as determined by two-dimensional mapping of their methionine-labeled tryptic peptides. Hamster cells transformed by various primate papovaviruses (simian virus 40, BK virus, and JC virus) synthesized indistinguishable Tau antigens, as determined by two-dimensional peptide mapping. When tested by the same procedure, these proteins and the ones made in monkey and human cells were found to be related to the Tau antigens isolated from simian virus 40-transformed mouse and rat cells. Based on these results, an "evolutionary tree" was constructed to show the relationship among the methionine-containing tryptic peptides of all of these proteins.
机译：在免疫沉淀后，在未感染的和Simian病毒40感染的猴细胞中检测到Tau抗原（也称为细胞或非血管肿瘤抗原），含有Simian病毒40诱导的肿瘤（抗-T血清）的免疫沉淀。将这两种蛋白质（56,000道尔顿）用各种量的葡萄球菌V8蛋白酶消化成类似大小的肽。从受感染的猴细胞中分离的Tau抗原与Simian病毒40转化的人细胞的相应蛋白质不相同，如通过其甲硫氨酸标记的胰蛋白酶肽的二维测绘所确定的。由各种灵长类动物乳头病毒（Simian病毒40，BK病毒和JC病毒）转化的仓鼠细胞合成了无法区分的Tau抗原，如二维肽测绘所确定的。当通过相同的程序测试时，发现这些蛋白质和用猴子和人细胞制备的蛋白质与来自Simian病毒40-转化的小鼠和大鼠细胞分离的Tau抗原有关。基于这些结果，构建了“进化树”以显示所有这些蛋白质的含甲硫氨酸的胰蛋白肽之间的关系。

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    《Journal of Virology》 |1980年第2期|共7页
  • 作者

    D T Simmons;

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  • 收录信息 美国《科学引文索引》(SCI);
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