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首页> 外文期刊>Journal of Virology >Restriction endonuclease mapping of unintegrated viral DNA of B- and N-tropic BALB/c murine leukemia virus.
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Restriction endonuclease mapping of unintegrated viral DNA of B- and N-tropic BALB/c murine leukemia virus.

机译：B-和N-热带BALB / C鼠白血病病毒未聚集的内切核酸酶映射。

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Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and analyzed by the Southern blot hybridization procedure. EcoRI did not cleave the two genomes. A physical map of 15 cleavage sites on B- and N-tropic genomes was constructed with the other restriction endonucleases. Identical cleavage sites of B- and N-tropic MuLV DNAs were found with all these enzymes. However, the N-tropic linear genome was found to lack about 75 base pairs at each end of the molecule. PstI, KpnI, and SmaI recognize a cleavage site at both ends of the linear molecules. And sequences derived from the 5' end of the RNA genome were found in the third left end of the linear DNA and at its extreme right-end terminus, suggesting the presence of redundant sequences. Two species of closed circular viral DNA were observed. The larger species has the same size as the linear molecule and appears to be a circularized form of linear DNA. The smaller species contains sequences common to both the linear and the larger circular viral DNA but seems to be deleted from sequences present at either one or both ends of the linear DNA. Therefore, the general structure of the linear and circular DNA species of these B- and N-tropic endogenous BALB/c MuLV appears analogous to the structure found with other retroviruses.
机译：从新感染的小鼠细胞中提取了B-和N-热带内源性BALB / C鼠白血病病毒（MULV）的未成复性线性和闭合圆形DNA，并用ECORI，XHOI，PVUI，HindIII，Sali，Xbai，KPNI，SMAI和PSTI限制性内切核酸酶。通过电泳分离DNA片段并通过Southern印迹杂交方法分析。 EcoRi没有切割两个基因组。用其他限制性内切核酸酶构建B-和N-热带基因组上的15个切割位点的物理图。用所有这些酶发现B-和N-热带MULV DNA的相同裂解位点。然而，发现N-热带线性基因组在分子的每一端缺乏约75个碱基对。 PSTI，KPNI和SMAI识别线性分子两端的裂解部位。源自RNA基因组的5'末端的序列在线性DNA的第三左端和其极端右端末端发现，表明存在冗余序列。观察到两种封闭的圆形病毒DNA。较大的物种具有与线性分子相同的大小，似乎是圆形的线性DNA形式。较小的物种含有与线性和较大的圆形病毒DNA共同常见的序列，但似乎被从存在于线性DNA的一端或两端的序列中删除。因此，这些B-和N-热带内源性BALB / C MULV的线性和圆形DNA种类的一般结构类似于用其他逆转录病毒发现的结构。

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