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首页> 外文期刊>Journal of Virology >Properties of Cells Carrying the Herpes Simplex Virus Type 2 Thymidine Kinase Gene: Mechanisms of Reversion to a Thymidine Kinase-Negative Phenotype
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Properties of Cells Carrying the Herpes Simplex Virus Type 2 Thymidine Kinase Gene: Mechanisms of Reversion to a Thymidine Kinase-Negative Phenotype

机译：携带疱疹病毒的细胞的性质2胸苷激酶基因：逆变对胸苷激酶阴性表型的逆转机制

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摘要

We have isolated cells with a thymidine kinase-negative (tk?) phenotype from cells which carry the herpes simplex virus type 2 tk gene by selection in 5-bromodeoxyuridine or 9-(2-hydroxyethoxymethyl)guanine. Both selection routines generated revertants with a frequency of 10?3 to 10?4, and resistance to either compound conferred simultaneous resistance to the other. tk? revertants fell into three classes: (i) cells that arose by deletion of all virus sequences, (ii) cells that had lost the virus tk gene but retained a nonselected virus-specific function and arose by deletion of part of the virus-specific sequence, and (iii) cells that retained the potential to express all of the virus-specific functions of the parental cells and retained all of the virus-specific DNA sequences.
机译：我们具有胸苷激酶阴性（TK β）的分离细胞，来自细胞的表型，通过选择在5-溴脱氧尿苷或9-（2-羟基乙氧基甲基）鸟嘌呤中携带疱疹病毒型2 TK基因。两个选择例程产生的频率为10 3 到10 Δ4，以及对任一化合物的耐受赋予另一个化合物的抗性。 TK ？回归分为三类：（i）通过缺失所有病毒序列而产生的细胞，（ii）丢失病毒Tk基因但保留了非选择性病毒特异性功能并通过缺失部分病毒特异性序列，（III）细胞保留了表达亲本细胞的所有病毒特异性功能并保留所有病毒特异性DNA序列。

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