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首页> 外文期刊>Journal of Virology >Virus-Specific RNA Synthesis in Cells Infected by Infectious Pancreatic Necrosis Virus
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Virus-Specific RNA Synthesis in Cells Infected by Infectious Pancreatic Necrosis Virus

机译：感染性胰腺坏死病毒感染细胞中的病毒特异性RNA合成

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Pulse-labeling experiments with [3H]uridine revealed that the rate of infections pancreatic necrosis virus-specific RNA synthesis was maximal at 8 to 10 h after infection and was completely diminished by 12 to 14 h. Three forms of RNA intermediates were detected: (i) a putative transcription intermediate (TRI) which comigrated in acrylamide gels with virion double-stranded RNA (dsRNA) after RNase treatment; (ii) a 24S genome length mRNA which could be resolved into two bands by polyacrylamide gel electrophoresis; and (iii) a 14S dsRNA component indistinguishable from virion RNA by gradient centrifugation and gel electrophoresis. The TRI (i) was LiCl precipitable; (ii) sedimented slightly faster and broader (14 to 16S) than the 14S virion dsRNA; (iii) had a lower electrophoretic mobility in acrylamide gels than dsRNA, barely entering acrylamide gels as a heterogenous component; (iv) yielded genome-sized pieces of dsRNA after RNase digestion; and (v) was the most abundant RNA form early in the infectious cycle. The 24S single-stranded RNA was thought to be the viral mRNA since it: (i) became labeled during short pulses; (ii) was found in the polysomal fraction of infected cells; and (iii) hybridized to denatured viral RNA, forming two segments of RNase-resistant RNA that comigrated with virion dsRNA in gels. The 24S mRNA component was formed before the synthesis of dsRNA, and radioactivity could be chased from 24S single-stranded RNA to dsRNA, indicating that 24S RNA may serve as template for the synthesis of complementary strands to form dsRNA. Similar to reovirus, infectious pancreatic necrosis viral 24S mRNA contained no polyadenylic acid tracts.
机译：用[ 3 h]尿苷的脉冲标记实验显示，感染后8至10小时的感染率胰腺坏死病毒特异性RNA合成最大化，并完全减少12至14小时。检测到三种形式的RNA中间体：（i）在RNase治疗后，用病毒素双链RNA（DSRNA）在丙烯酰胺凝胶中调用的推定转录中间体（TRI）; （ii）24S基因组长度mRNA，可通过聚丙烯酰胺凝胶电泳分解成两个带; （iii）通过梯度离心和凝胶电泳，从病毒杆菌RNA中难以区分的14s DsRNA组分。 TRI（i）是LICL可见的; （ii）略微沉淀，比14s virion dsrna更快，更广泛（14至16s）; （iii）在丙烯酰胺凝胶中的电泳迁移率低于DSRNA，几乎没有进入丙烯酰胺凝胶作为异源组分; （iv）在RNase消化后产生基因组大小的dsRNA; （v）在传染性周期早期形成最丰富的RNA。 24s单链RNA被认为是其中的病毒mRNA：（i）在短脉冲期间被标记; （ii）发现在感染细胞的多粒组成部分中; （iii）与变性病毒RNA杂交，形成与凝胶中的VIRION DSRNA的抗rnase抗性RNA的两个区段。在合成DsRNA之前形成24S mRNA组分，并且可以将放射性从24秒的单链RNA螯合到DSRNA，表明24s RNA可以用作合成互补链以形成dsRNA的模板。类似于再遗治，传染性胰腺坏死病毒24s mRNA没有多腺苷粉。

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