The role of the basic N-terminal region of protein L18 in 5S RNA–23S RNA complex formation

Roger A. Garrett; Veronica Newberry

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The role of the basic N-terminal region of protein L18 in 5S RNA–23S RNA complex formation

机译：蛋白质L18基本N-末端区域在5S RNA-23S RNA复合物中的作用

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Of the three proteins, L5, L18 and L25, which bind to 5S RNA, the former two effect the interaction of 5S RNA with 23S RNA. We have used trypsin as a probe to investigate the roles of the proteins in this RNA-RNA assembly, with the following results: (1) In complexes with 5S RNA, the highly basic N-terminal region of L18 is accessible to trypsin. This accessibility is unaffected by L25. However, its presence is essential for stimulating L5 binding. (2) In 5S RNA-protein-23S RNA complexes proteins L5 and L18 are both strongly resistant to proteolysis. (3) No 5S RNA-23S RNA complex formation occurs in the presence of L5 and the C-terminal Ll8 fragment. Two possible models for the mechanism of RNA-RNA assembly are proposed.

机译：在结合5S RNA的三种蛋白质，L5，L18和L25中，前两者效果5S RNA与23S RNA的相互作用。我们使用胰蛋白酶作为探针，以研究该RNA-RNA组件中蛋白质的作用，其中：（1）在具有5S RNA的络合物中，胰蛋白酶可以获得L18的高碱性N-末端区域。此可访问性不受L25的影响。然而，其存在对于刺激L5结合至关重要。（2）在5S中，RNA-蛋白-23S RNA络合物蛋白质L5和L18均耐受蛋白水解。（3）在L5和C末端LL8片段存在下，没有5S RNA-23S RNA复合物形成。提出了用于RNA-RNA组件的两个可能模型。

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《Nucleic acids research》 |1980年第18期|共12页
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Roger A. Garrett; Veronica Newberry;
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收录信息美国《科学引文索引》(SCI);
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1. The role of the basic N-terminal region of protein L18 in 5S RNA–23S RNA complex formation [J] . Roger A. Garrett, Veronica Newberry Nucleic acids research . 1980,第18期

机译：蛋白质L18基本N-末端区域在5S RNA-23S RNA复合物中的作用
2. The role of the basic N-terminal region of protein L18 in 5S RNA–23S RNA complex formation [J] . Roger A. Garrett, Veronica Newberry Nucleic acids research . 1980,第18期

机译：蛋白质L18基本N-末端区域在5S RNA-23S RNA复合物中的作用
3. The Thermus thermophilus 5S rRNA -Protein Complex: Identification of Specific Binding Sites for Proteins L5 and L18 in the 5S rRNA [J] . G.M.Gongadze, Institute of Protein Research Russian Academy of Sciences Pushchino Moscow Region 142290 Russia, A.A.Perederina, Molecular Biology . 2001,第4期

机译：Thermus thermophilus 5S rRNA-蛋白质复合物：5S rRNA中蛋白质L5和L18的特异性结合位点的鉴定
4. Identification of an Alga-Lysing bacteria isolated from TaiHu Lake by the analysis of 16S rRNA and 16S-23S rRNA intergenic spacer region [C] . LI Yun-hui, PU Yue-pu, YIN Li-hong International Forum on Progress on Post-Genome Technologies(5'IFPT); 20070910-11; Suzhou(CN) . 2007

机译：通过16S rRNA和16S-23S rRNA基因间隔区分析鉴定从太湖分离的溶藻细菌。
5. Study of common Vibrio 5S ribosomal RNA sequence variants and development of a new methodology to identify the phenotypes of Vibrio proteolyticus 5S rRNA variants. [D] . Zhang, Zhengdong. 2002

机译：常见的弧菌5S核糖体RNA序列变异体的研究和一种新方法的开发，以鉴定蛋白水解弧菌5S rRNA变异体的表型。
6. The role of the basic N-terminal region of protein L18 in 5S RNA-23S RNA complex formation. [O] . V Newberry, R A Garrett 1980

机译：蛋白质L18的基本N末端区域在5S RNA-23S RNA复合物形成中的作用。
7. The role of the basic N-terminal region of protein L18 in 5S RNA-23S RNA complex formation. [O] . Newberry, V, Garrett, R A 1980

机译：蛋白质L18的基本N末端区域在5S RNA-23S RNA复合物形成中的作用。

The role of the basic N-terminal region of protein L18 in 5S RNA–23S RNA complex formation

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