2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides

D. Ferbus; J. Justesen; M.N. Thang

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2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides

机译：2'5'obgoocenylate合成酶，干扰素诱导酶：用于产物的直接测定方法，2'5'OHgoadenylate和2'5'共寡核苷酸

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The interferon induced enzyme 2′5′ oligoadenylate synthetase produces 2′5′ pppA(pA)_n the first discovered natural nucleotide with a 2′5′ linkage. We describe a direct assay of this enzyme based on separation by thin layer chromatography (TLC) of the substrate ATP and the products 2′5′pppA(pA)_n (n ≥ 1). This technique presents obvious advantages compared to the currently used methods. Moreover the enzyme uses other nucleotides as substrates forming co-oligonucleotides 2′5′pppA(pA)_n pN (N= U,G,C,dA,dG,dT and dC). Additional procedures are described using different developing solvent systems for the separation of the core-2′5′ oligonucleotides (2′5 A(pA)_npN) containing AMP-residues entirely and those with another nucleotide at the 2′end.

机译：干扰素诱导的酶2'5'5'LIGOONYLATE合成酶产生2'5'PPPA（PA）_{n 第一个发现的天然核苷酸与2'5'连锁。我们基于基于底物ATP的薄层色谱（TLC）和产物2'5'PPPA（PA）_{N （n≥1）的分离，描述该酶的直接测定。与目前使用的方法相比，该技术具有明显的优势。此外，酶使用其他核苷酸作为形成副寡核苷酸的底物2'5'ppPa（PA）_{n pn（n = u，g，c，da，dg，dt和dc）。使用不同的显影溶剂系统来描述用于分离核 - 2'5'寡核苷酸（2'5a（PA）寡核苷酸的分离（2'5a（PA）_{n pn）完全含有AMP-残基的寡核苷酸和与另一个核苷酸的寡核苷酸的分离2'end。}}}}

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《Nucleic acids research》 |1980年第14期|共14页
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D. Ferbus; J. Justesen; M.N. Thang;
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1. 2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides [J] . D. Ferbus, J. Justesen, M.N. Thang Nucleic acids research . 1980,第14期

机译：2'5'obgoocenylate合成酶，干扰素诱导酶：用于产物的直接测定方法，2'5'OHgoadenylate和2'5'共寡核苷酸
2. 2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides [J] . D. Ferbus, J. Justesen, M.N. Thang Nucleic acids research . 1980,第14期

机译：2'5'obgoocenylate合成酶，干扰素诱导酶：用于产物的直接测定方法，2'5'OHgoadenylate和2'5'共寡核苷酸
3. Accurate Detection of Adenylation Domain Functions in Nonribosomal Peptide Synthetases by an Enzyme-linked Immunosorbent Assay System Using Active Site-directed Probes for Adenylation Domains [J] . Ishikawa Fumihiro, Miyamoto Kengo, Konno Sho, ACS Chemical Biology . 2015,第12期

机译：酶联免疫吸附测定系统使用腺苷酸化域活性位点探针精确检测非核糖体肽合成酶中腺苷酸化域功能
4. A method to extract spots from the image of the ELISA (enzyme-linked immunosorbent assay) spot assay [C] . Chih-Yang Lin, Yu-Tai Ching, Wu-Hsieh, Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE . 2001

机译：一种从ELISA图像中提取斑点的方法（酶联免疫吸附测定）
5. Development of an indirect enzyme-linked immunosorbent assay for the quantification of inducible nitric oxide synthase protein (iNOS). [D] . Heaton, Phillip R. 2005

机译：间接酶联免疫吸附测定技术的发展，用于定量诱导型一氧化氮合酶蛋白（iNOS）。
6. 25 oligoadenylate synthetase an interferon induced enzyme: direct assay methods for the products 25 oligoadenylates and 25 co-oligonucleotides. [O] . J Justesen, D Ferbus, M N Thang 1980

机译：25寡腺苷酸合成酶一种干扰素诱导的酶：产品25寡腺苷酸和25共寡核苷酸的直接测定方法。
7. Methods for the Determination of the Interferon-Induced Enzyme 2'-5' Oligoadenylate Synthetase in Mononuclear Blood Cells [O] . Bruchelt, G., Beck, J., Schilbach-Stückle, Karin, 1987

机译：测定单核细胞中干扰素诱导酶2'-5'寡聚腺苷酸合成酶的方法
8. UBCH8 Ubiquitin E2 Enzyme is Also the E2 Enzyme for ISG15, an Interferon Alpha/Beta Induced Ubiquitin-Like Protein. [R] . Krug, R. M., Zhao, C., Hulbregtse, J. 2004

机译：UBCH8泛素E2酶也是IsG15的E2酶，IsG15是一种干扰素α/β诱导的泛素样蛋白。

2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides

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