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首页> 外文期刊>Journal of Virology >Viral hemorrhagic septicemia of rainbow trout: selection of a thermoresistant virus variant and comparison of polypeptide synthesis with the wild-type virus strain.
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Viral hemorrhagic septicemia of rainbow trout: selection of a thermoresistant virus variant and comparison of polypeptide synthesis with the wild-type virus strain.

机译：虹鳟鱼病毒出血性杂皮虫：野生型病毒株的热静脉病毒变体选择及多肽合成的比较。

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摘要

Serial passage of viral hemorrhagic septicemia virus at gradually increasing temperature selected for a variant virus that replicates at 25 degrees C and has a low pathogenicity for rainbow trout. Viral hemorrhagic septicemia virus-specific polypeptide synthesis was examined in epithelioma papulosum cyprini cells infected with either a wild-type strain or a thermoresistant variant. The wild-type N and M1 proteins were synthesized throughout the course of infection, whereas L, G, and M2 were more actively translated later in the replication cycle. The wild-type strain was more cytotoxic at 25 than at 14 degrees C despite the fact that no translation could be evidenced when the temperature was raised. When epithelioma papulosum cyprini cells were infected with the variant virus, the kinetic study was obstructed since protein synthesis was difficult to observe by the pulse method at a low multiplicity of infection and aborted when the multiplicity of infection was raised. The variant was less cytotoxic at 25 degrees C than wild-type virus.
机译：病毒出血性败血症病毒在逐渐增加的温度下选择的序列通过，该温度为25摄氏度重复的变体病毒，对虹鳟鱼具有低致病性。在感染野生型菌株或热阻变体的上皮瘤骨髓内细胞中检查了病毒出血性遗体血症病毒特异性多肽合成。在整个感染过程中合成野生型N和M1蛋白，而L，G和M2更稍后在复制循环中更加积极地翻译。野生型菌株在25的细胞毒性比在14℃下更具细胞毒性，尽管在升温时可以证明翻译。当上皮瘤骨髓瘤Cyprini细胞感染变体病毒时，动力学研究被阻塞，因为蛋白质合成在低多样性感染时难以观察到脉冲法，并且当升高多个感染时中止。该变体比野生型病毒在25℃下的细胞毒性较少。

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