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首页> 外文期刊>Journal of Virology >Interferon-induced proteins in human fibroblasts and development of the antiviral state.
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Interferon-induced proteins in human fibroblasts and development of the antiviral state.

机译：干扰素诱导的人成纤维细胞中的蛋白质和抗病毒态的发育。

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Treatment of human fibroblasts with interferon induces the synthesis of several proteins, as detected by incorporation of [35S]methionine followed by analysis of cell extracts by polyacrylamide gel electrophoresis. The induction of these proteins had features in common with the development of the antiviral effect of interferon, such as (i) sensitivity to actinomycin D and cycloheximide when these compounds were added together with interferon, (ii) insensitivity to actinomycin D if the actinomycin D was added 2 h after the addition of interferon, (iii) similar dependence on interferon concentration, and (iv) species specificity for interferon. When interferon treatment was given in the presence of cycloheximide and actinomycin D was added before the removal of cycloheximide, all four proteins were induced, thus suggesting that their inductions are coordinated. Labeling for 2-h periods at varying time intervals after the addition of interferon revealed that the synthesis of these proteins was induced within a few hours, peaked at different time intervals, and was soon followed by a marked decline, suggesting that the mRNA's for these proteins have short half-lives. Moreover, this decline occurred despite the fact that the cells were continuously exposed to interferon, and there was no measurable loss of interferon activity in the medium. This suggests that the induction of these proteins is transient and is apparently subject to further control.
机译：用干扰素治疗人成纤维细胞诱导几种蛋白质的合成，如通过掺入[35s]甲硫氨酸的检测，然后通过聚丙烯酰胺凝胶电泳分析细胞提取物。这些蛋白质的诱导与干扰素的抗病毒效果的发展共同的特征，例如（i）当这些化合物与干扰素一起加入这些化合物时对放线霉素D和环己酰亚胺的敏感性，如果放素霉素D对放射素D的不敏感性添加干扰素后2小时，（iii）对干扰素浓度的类似依赖性，（IV）干扰素的特异性。当在除去环己酰亚胺的存在下，在除去环己酰亚胺之前给出干扰素处理时，诱导所有四种蛋白质，因此表明它们的诱导是协调的。在添加干扰素后，在不同时间间隔标记2-H周期显示这些蛋白质的合成在几小时内诱导，以不同的时间间隔达到峰值，并且很快被显着下降，这表明MRNA为此蛋白质有短的半衰期。此外，尽管细胞连续暴露于干扰素，但这种下降发生了这种下降，并且在培养基中没有可测量的干扰素活性丧失。这表明这些蛋白质的诱导是短暂的，并且显然是有进一步的对照。

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