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首页> 外文期刊>Journal of Virology >Mammary tumor virus proviral DNA in normal murine tissue and non-virally induced mammary tumors.
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Mammary tumor virus proviral DNA in normal murine tissue and non-virally induced mammary tumors.

机译：乳腺肿瘤病毒在普通鼠组织和非易生诱导的乳腺肿瘤中的脱脂DNA。

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The Southern DNA filter transfer technique was used to study the involvement of the endogenous mouse mammary tumor virus (MMTV) in the development of mammary tumors of nonviral etiology. The presence of extra MMTV proviruses in the genomes of these non-virally induced mammary tumors would indicate an integration of the provirus of an activated endogenous MMTV. Acquisition of MMTV proviruses did not seem to be an absolute requirement for the development of hormone or carcinogenically induced mammary tumors in strain BALB/c nor for hormone-induced mammary tumors in mouse strains 020, C57BL, and C3Hf. In some hormone-induced mammary tumors we did observe extra MMTV proviruses in submolar quantities, indicating that reintegration may occasionally occur and that only a part of the tumor cells acquired new MMTV DNA information. Hormone-dependent and -independent primary mammary tumors of the mouse strain GR, which are controlled by the Mtv-2 mammary tumor induction gene, all acquired extra MMTV proviruses. Most of these extra MMTV proviral-DNA-containing fragments appeared present in submolar quantities, suggesting that only part of the tumor cells acquired extra MMTV proviral information. These findings indicate that the initially transformed mammary gland cells of non-virally induced mammary tumors do not necessarily acquire extra MMTV proviral DNA information, in contrast to the MMTV-induced mammary tumors, in which all tumor cells contain extra MMTV DNA information.
机译：Southern DNA过滤器转移技术用于研究内源小鼠乳腺肿瘤病毒（MMTV）在非血管病因乳腺癌发育中的累积。在这些非公有诱导的乳腺肿瘤的基因组中存在额外的MMTV潜水血管，这将表明活化内源性MMTV的潜水员的整合。收购MMTV潜水术似乎并不是对菌株BALB / C中的激素或泌尿根诱导的乳腺肿瘤的产生绝对要求，也不是小鼠菌株020，C57BL和C3HF中的激素诱导的乳腺肿瘤。在一些激素诱导的乳腺肿瘤中，我们确实观察到潜水量的额外MMTV潜水术，表明可以偶尔发生重新融入，并且只有一部分肿瘤细胞获得了新的MMTV DNA信息。由MTV-2乳腺肿瘤诱导基因控制的小鼠菌株GR的激素依赖性和依赖性初级乳腺肿瘤，全部获得额外的MMTV潜水术。这些含额外的MMTV荧光-DNA的片段中的大多数出现在潜水量中存在，表明只有部分肿瘤细胞获得了额外的MMTV拓展信息。这些发现表明，初始转化的非病毒诱导的乳腺肿瘤的乳腺细胞不一定与MMTV诱导的乳腺肿瘤相比，所有肿瘤细胞含有额外的MMTV DNA信息的巨大MMTV荧光DNA信息。

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