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首页> 外文期刊>Journal of Virology >Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells.
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Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells.

机译：70S聚脲酰基聚合酶的表征从口蹄疫感染病毒感染细胞中分离的。

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摘要

A polyuridylic acid polymerase complex isolated from foot-and-mouth disease virus-infected cells sedimented at 70S in a sucrose gradient and appeared in the exclusion volume of an agarose column whose molecular weight cutoff was 5 x 10(6). Phenol extraction of the complex yielded a heterogeneous band of virus-specific RNA and an apparently host cell-derived 4.5 to 5S RNA, both of which are essentially single stranded. Neither RNA served as a template in the cell-free enzyme reaction. Polyacrylamide gel analysis revealed five polypeptides with molecular weights of 50,000, 56,000, 60,000, 70,000, and 74,000 and with molar ratios of 1:2:2:1:1, respectively. Autoradiography showed P56 to be the only major virus-induced polypeptide; the other proteins are apparently of host cell origin. Electron microscopic examination suggested a cartwheel shape for the polymerase complex which was seen to dissociate as polyadenylic acid was added. Antibody previously shown to inhibit enzyme activity aggregated the 70S units.
机译：从蔗糖梯度的70s沉积在70s的脚口疾病病毒感染细胞中分离的聚亚乙酸聚合酶复合物，并出现在分子量截止值为5×10（6）的琼脂糖柱的排除体积中。复合物的酚萃取产生了异质的病毒特异性RNA，并且明显宿主细胞衍生的4.5至5S rNA，两者都基本上滞留。既不是无细胞酶反应中的RNA也不用作模板。聚丙烯酰胺凝胶分析揭示了五种多肽，分子量为50,000,56,000,60,000,70,000和74,000，分别为1：2：2：1：1的摩尔比。 autoradoography显示p56是唯一主要的病毒诱导的多肽;其他蛋白质显然是宿主细胞来源。电子显微镜检查表明，作为加入聚腺苷酸的聚合酶复合物的卡车形状被视为解离。以前所示以抑制酶活性的抗体聚集70s单元。

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    《Journal of Virology》 |1981年第3期|共9页
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    J Polatnick; S Wool;

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  • 收录信息 美国《科学引文索引》(SCI);
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