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首页> 外文期刊>Journal of Virology >XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome.
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XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome.

机译：Xbai，Psti和Bglii限制酶图的毒氏菌 - 带状疱疹病毒基因组的两个取向。

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摘要

Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the XbaI and the BglII patterns, four 0.5 M fragments were identified. The arrangement of the fragments was determined by molecular hybridization techniques, and the terminal fragments were identified by lambda exonuclease digestion. The 0.5 M fragments, of which two were located at the same terminus of the genome, contained repeated sequences: one terminally and one inverted internally. These results were in agreement with the existence of two equimolar subpopulations of the varicella-zoster virus genome, differing in the relative orientation of a short region of unique sequences. This region was bounded by the repeated sequences. From the molecular weights of the submolar fragments, a maximal molecular weight of 5 x 10(6) for the repeated region and a minimal molecular weight of 3.5 x 10(6) for the short unique sequence could be calculated.
机译：用限制性内切核酸酶PSTI，XBAI和BGLII切割种子果实 - 带状病毒DNA，分别为18,22和20次碎片。基于这些碎片的分子量和摩尔，可以针对VariCella-Zoster病毒基因组计算84×10（6）的分子量。在XBAI和BGLII模式中，鉴定了四种0.5M片段。片段的布置是通过分子杂交技术确定的，并且通过λ外切核酸酶消化鉴定末端片段。 0.5M片段，其中两个位于基因组的相同末端，含有重复序列：一个终端，一个末端，在内部倒置。这些结果与存在的毒氏菌 - 带状疱疹病毒基因组的两种等摩尔群存在一致，不同于独特序列的短区域的相对取向。该区域被重复序列界定。从潜水器片段的分子量，可以计算重复区域的最大分子量为5×10（6），并且可以计算短独特序列的最小分子量为3.5×10（6）。

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