• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The biochemical journal >[14C]acetylcholine synthesis and [14C]carbon dioxide production from [U-14C]glucose by tissue prisms from human neocortex
【24h】

[14C]acetylcholine synthesis and [14C]carbon dioxide production from [U-14C]glucose by tissue prisms from human neocortex

机译：[14c]乙酰胆碱合成和[14c]通过来自人Neocortex的组织棱镜的[U-14C]葡萄糖产生的二氧化碳产生

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

p1. [14C]Acetylcholine synthesis and 14CO2 production from [U-14C]glucose has been measured in tissue prism preparations from human neocortex. 2. Electron micrographs of prisms from human and rat neocortex show that both contain intact synaptic endings with evenly-distributed vesicles and normal-appearing mitochondria, but only poorly preserved cell body structure. 3. Synthesis of [14C]acetylcholine in prisms from rat neocortex is similar to estimates for turnover in vivo. Synthesis in prisms from human neocortex is 18% of that in rat tissue and 64% of that in tissue from baboon neocortex for incubations performed in 31 mM-K+. 4. Investigations of prisms prepared from rat brains stored at 37 degrees C after death revealed that synthesis of [14C]acetylcholine in the presence of 31 mM-K+ was greatly decreased within 30 min of post-mortem incubation, whereas synthesis at 5 mM-K+ and production of 14CO2 at both K+ concentrations were only significantly affected after longer periods. Changes were similar in neocortex and striatum. Thus human autopsy material is unlikely to be suitable for use with this system. 5. Investigations using animal models suggest that [14C]acetylcholine synthesis and 14CO2 production are not affected by surgical or anaesthetic procedures. 6. Neither [14C]acetylcholine synthesis nor 14CO2 production in human prisms was significantly changed with age between 15 and 68 years. 7. Samples from patients with the dementing condition Alzheimer9s disease showed a significant decrease in [14C]acetylcholine synthesis to 47% of normal samples and a significant increase of 39% in production of 14CO2./p
机译：> 1。 [14C]乙酰胆碱合成和14CO2从[U-14C]葡萄糖的产生已从人Neocortex的组织棱镜制剂中测量。 2.来自人和大鼠Neocortex的棱镜的电子显微照片显示，两者都含有完整的突触结束，含有均匀分布的囊泡和正常出现的线粒体，但仅保存不良的细胞体结构。 3.大鼠Neocortex的棱氮中[14C]乙酰胆碱的合成类似于体内营业额的估计。来自人Neocortex的棱镜的合成是大鼠组织中的18％，其中64％的64％来自狒狒Neocortex的组织中，以在31mm-k +中进行。 4.在死亡后，从37摄氏度储存的大鼠大脑制备的棱镜的调查显示[14C]乙酰胆碱在验疗后30分钟内大大降低，而5mm-in合成K +和K +浓度的14Co2的产生仅在更长的时间内显着影响。 Neocortex和纹状体的变化相似。因此，人尸检材料不太可能适用于该系统。 5.使用动物模型的调查表明[14C]乙酰胆碱合成和14CO2生产不受外科或麻醉程序的影响。 6.乙酰胆碱合成和14Co2人类棱镜的产生，15至68岁之间的年龄明显变化。 7.来自乳剂状况的样品Alzheimer9s疾病显示[14C]乙酰胆碱合成的显着降低至正常样品的47％，并在14CO2的产生中显着增加39％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号