The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver

B R Rabin; M B Cooper; M R Estall

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The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver

机译：使用蛋白酶以确定细胞色素p-450在大鼠肝脏光滑内质网的囊泡中的细胞色素p-450的拓扑位置

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p1. The phospholipid bilayer of intact vesicles from smooth endoplasmic reticulum is impermeable to macromolecules. Specific and non-specific proteinases were used to investigate the site of membrane proteins in the transverse plane of the bilayer. 2. When two proteinases were used in conjunction, denaturing effects additional to proteolysis were observed on cytochrome P-450 content and glucose 6-phosphatase activity which did not depend on the integrity of the phospholipid bilayer. 3. When lipid peroxidation was inhibited, these effects were not observed./p

机译：> 1。来自光滑的内质网的完整囊泡的磷脂双层是不可渗透的大分子。使用特异性和非特异性蛋白酶来研究双层横向平面中的膜蛋白位点。 2.当结合使用两种蛋白酶时，在细胞色素P-450含量和葡萄糖6-磷酸酶活性上观察到蛋白水解的变性效果，其不依赖于磷脂双层的完整性。 3.当抑制脂质过氧化时，未观察到这些效果。

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《The biochemical journal》 |1981年第2期|共页
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B R Rabin; M B Cooper; M R Estall;
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收录信息美国《科学引文索引》(SCI);
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1. The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver [J] . B R Rabin, M B Cooper, M R Estall The biochemical journal . 1981,第2期

机译：使用蛋白酶以确定细胞色素p-450在大鼠肝脏光滑内质网的囊泡中的细胞色素p-450的拓扑位置
2. The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver [J] . B R Rabin, M B Cooper, M R Estall The biochemical journal . 1981,第2期

机译：使用蛋白酶以确定细胞色素p-450在大鼠肝脏光滑内质网的囊泡中的细胞色素p-450的拓扑位置
3. Asymmetric distribution of cytochrome P-450 and NADPH–cytochrome P-450 (cytochrome c) reductase in vesicles from smooth endoplasmic reticulum of rat liver [J] . Brian R. Rabin, John A. Craft, Margaret R. Estall, The biochemical journal . 1980,第3期

机译：大鼠肝脏平滑内质网囊泡中细胞色素P-450和NADPH–细胞色素P-450（细胞色素c）还原酶的不对称分布
4. Effects of Radix Total Glucosides of Paeonia on the Endoplasmic Reticulum Stress and Macrophage Recruitment on Diabetic Cardiomyopathy Rats [C] . Hongyu Kang, Ximin Liu, Yaping Zheng, International Conference on Materials Engineering and Information Technology Applications . 2017

机译：芍药的含量对糖尿病心肌病大鼠内质网胁迫和巨噬细胞募集的影响
5. In vivo and in vitro comparison of cytochrome P-450 expression and DNA adduct formation in rat liver and lung treated with benzo(a)pyrene. [D] . Hand, Jeanine Ann. 2001

机译：体内和体外比较用苯并（a）treated处理的大鼠肝和肺中细胞色素P-450表达和DNA加合物的形成。
6. The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver. [O] . M B Cooper, M R Estall, B R Rabin 1981

机译：蛋白酶用于确定细胞色素P-450在大鼠肝脏平滑内质网来源的囊泡中的拓扑位置。
7. The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver. [O] . Cooper, M B, Estall, M R, Rabin, B R 1981

机译：蛋白酶用于确定细胞色素P-450在大鼠肝脏平滑内质网来源的囊泡中的拓扑位置。

The use of proteinases to determine the topological location of cytochrome P-450 in vesicles derived from smooth endoplasmic reticulum of rat liver

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